Swimming reduces fatty acids-associated hypothalamic damage in mice

Publication date: Available online 11 November 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Pedro Augusto Silva Nogueira, Miriam Pimenta Pereira, Jeferson José Gomes Soares, Juliana de Assis Silva Gomes, Daniele Lisboa Ribeiro, Daniela Soares Razolli, Licio Augusto Velloso, Morun Bernardino Neto, Renata Graciele ZanonAbstractThe arcuate and the paraventricular and lateral hypothalamic nuclei, related to hunger and satiety control, are generally compromised by excess fatty acids. In this situation, fatty acids cause inflammation via TLR4 (toll like receptor 4) and the nuclei become less responsive to the hormones leptin and insulin, contributing to the development of obesity. In this work, these nuclei were analyzed in animals fed with high-fat diet and submitted to swimming without and with load for two months. For this, frontal sections of the hypothalamus were immunolabelled with GFAP (glial fibrillary acidic protein), synaptophysin, IL-6 (interleukin 6) and TLR4. Also, proteins extracted from the hypothalamus were analyzed using Western blotting (GFAP and synaptophysin), fluorometric analysis for caspases 3 and 7, and CBA (cytometric bead array) for Th1, Th2, and Th17 profiles. The high-fat diet significantly caused overweight and, in the hypothalamus, decreased synapses and increased astrocytic reactivity. The swimming with load, especially 80% of the maximum load, reduced those consequences. The high-fat diet increased TLR4 in the arcuate nucleus and the swimm...
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research