Genotypic divergence of Avibacterium paragallinarum isolates with different growth requirements for nicotinamide adenine dinucleotide.

Genotypic divergence of Avibacterium paragallinarum isolates with different growth requirements for nicotinamide adenine dinucleotide.
Avian Pathol. 2019 Nov 11;:1-34
Authors: Jeong OM, Kang MS, Blackall PJ, Jeon BW, Kim JH, Jeong J, Lee HJ, Kim DW, Kwon YK, Kim JH
Abstract
In 2017, for the first time in Asia, we reported the isolation of variants of Avibacterium paragallinarum with atypical NAD dependency. The present study was conducted to characterize the genotypes of 24 isolates of Av. paragallinarum in Korea, including the four variants reported previously. Most of the typical isolates (19/20) showed a unique ERIC-PCR pattern with no ERIC-PCR patterns in common between the typical isolates and the variants. Furthermore, the variants shared no ERIC-PCR patterns among themselves. All the typical NAD-dependent isolates belonged to the same phylogenetic group based on both 16S rRNA and hagA gene sequences. The four variants were placed in several groups distinct from the typical isolates. In the 16S rRNA phylogenetic analysis, two of the variants were not closely aligned to all other Av. paragallinarum, although they were clearly members of the genus Avibacterium. The other variants were clustered together with NAD atypical isolates from geographically diverse global locations. Compared with the Modesto reference strain AY499870, all the variants lacked a TTTTT stretch at positions 182-186 in the 16S rRNA gene and the same deletion ...

Source: Avian Pathology - November 10, 2019 Category: Pathology Authors: Jeong OM, Kang MS, Blackall PJ, Jeon BW, Kim JH, Jeong J, Lee HJ, Kim DW, Kwon YK, Kim JH Tags: Avian Pathol Source Type: research