Evaluation of Anyplex MTB/NTM Test for The Detection of Mycobacterium tuberculosis in Clinical Specimens.
[Evaluation of Anyplex MTB/NTM Test for The Detection of Mycobacterium tuberculosis in Clinical Specimens]. Mikrobiyol Bul. 2019 Oct;53(4):355-363 Authors: Alp A, Sarıbaş Z Abstract One of the most important steps for the control of tuberculosis is rapid and accurate detection of Mycobacterium tuberculosis in clinical samples. The early and accurate diagnosis of tuberculosis allows the initiation of the effective treatment regimen as early as possible. However the early diagnosis of tuberculosis can be achieved by the integration of molecular methods into the diagnostic algorithm of tuberculosis together with the gold standard culture methods. For this reason, molecular methods have become valuable diagnostic tools in routine diagnostic laboratories in recent years. The aim of this study was to determine the diagnostic efficacy of Anyplex MTB/NTM test (Seegene, South Korea) used for the molecular diagnosis of tuberculosis in routine molecular diagnostic laboratories. In addition to this aim, a preliminary evaluation of in-house polymerase chain reaction (PCR) primers that was designed to produce a kit as an alternative against imported commercial kits was performed. Ten thousand six hundred fifthy two clinical specimens that were collected from suspected tuberculosis cases in three years were included in the study. All samples were tested by microscopic examination after staining, culture and real-time PCR (Rt-PCR) methods. The smears were examined by m...
ConclusionsIn elderly patients, inflammation level and physical activity level, along with initial smear grade may have a significant impact on delayed sputum conversion. Non ‐conversion after two months of treatment might not be related with mortality.
Conclusion: Multiplex PCR using MPB 64 and IS6110 primers has a high sensitivity and specificity in diagnosis of FGTB. PMID: 31801325 [PubMed - in process]
ConclusionA high suspicion level for TB, the early diagnosis and the prompt initiation of therapy could increase the survival rates among SOT patients. Overall, renal and lung TRs appear to have a higher predisposition for acquiring TB than other type of recipients. Monitoring of the high-risk recipients, prompt diagnosis, and appropriate treatment are required to manage TB infection among TRs especially in endemic areas.
Conclusions: The results show that intricate TB cases were notified by the local health services. Strategies of surveillance and articulation with these health services seem to contribute to the identification of TB cases among PDL.
We report the first evaluation of clinical specimens with REFtb assays in comparison to the gold standards for tuberculosis diagnosis, culture and smear microscopy. REFtb assays allowed diagnosis of 160 patients from 16 different countries with a sensitivity of 89% for smear-positive, culture-positive samples and 88% for smear-negative, culture-positive samples with a specificity of 82%. The negative predictive value of REFtb for tuberculosis infection is 93%, and the positive predictive value is 79%. Overall, these data point toward the need for larger accuracy studies by third parties using a commercially available REFtb...
Conclusion: social vulnerable and comordid IDUs have high risk for TB. They should be screened annually and have an individualized approach for preventing low treatment outcome.
Conclusion: Pulmonary TB has negative impact on male sexual function. Thus, sexual problems should be in mind during assessment and evaluation of patient with tuberculosis.
Background: Diabetes mellitus (DM) has an influence on many aspects of tuberculosis (TB).Objectives: to correlate the influence of diabetes mellitus on sputum conversion rate and antituberculous drug resistance.Methods: The study comprised 194 pulmonary tuberculosis (PTB) patients divided in two groups, i.e. the group of TB associated with DM and the group of patients affected only by TB. Sputum smear and liquid culture (MGIT 960) examination were performed at TB diagnosis and subsequently at completion of the initial phase as well as during the continuation phase of TB treatment. Drug sensitivity test (DST) was also perfo...
Conclusion: Patient with ABPA frequently received empiric ATT as smear –ve TB in high TB burden country. This results in over diagnosis of TB and unnecessary use of global resource. Moreover dealy in diagnosis leads to long term complications. Awareness regarding ABPA is required in high TB burden countries.