Investigation of Polymerase Chain Reaction Method in Patients with Suspected Chronic Cutaneous Leishmania of Negative Microscopy.

[Investigation of Polymerase Chain Reaction Method in Patients with Suspected Chronic Cutaneous Leishmania of Negative Microscopy]. Mikrobiyol Bul. 2019 Oct;53(4):408-418 Authors: Çulha G, Kaya T, Gülbol Duran G, Urhan Küçük M, Doğramacı AÇ, Tiyekli Çelik D Abstract Leishmaniasis is a parasitic disease that is transmitted to humans by the bites of infected female phlebotomine sandflies. In the diagnosis of cutaneous leishmaniasis (CL), in the smear samples, the demonstration of the parasite by microscope remains a gold standard method. However, it becomes difficult to diagnose the parasite since the number of amastigotes in chronic cases with a lesion of one year or longer is very low. Due to many factor such as patients primarily do not to take any notice these lesions in their bodies, do not apply to health institutions or late applied, receive wrong treatment; the diagnosis and treatment are delayed. In addition, it is been worse prognosis by add secondary infection to lesions and wounds become chronic. For this reason, molecular methods are used in addition to microscopic examination in chronic suspected CL cases. It was aimed to reveal of the molecular diagnostic value in chronic suspected CL cases by polymerase chain reaction (PCR) in the smear belonging to Turkish patients that reported to be evaluated clinically because it can not be seen Leishmania amastigotes in microscopic examination. Smear of 50 Tu...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

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Date: Tuesday, 01 07, 2020; Speaker: Daniel Neafsey, Assistant Professor, Harvard T.H. Chan School of Public Health; 5625 Fishers Lane; 5th Floor Conference Room
Source: NIH Calendar of Events - Category: American Health Source Type: events
Publication date: Available online 10 December 2019Source: Fish &Shellfish ImmunologyAuthor(s): Ze-Quan Mo, Xue-Li Lai, Wan-Tao Wang, Hong-Ping Chen, Zhi-Chang He, Rui Han, Jiu-Le Wang, Xiao-Chun Luo, Yan-Wei Li, Xue-Ming DanAbstractC-Raf proto-oncogene serine/threonine kinase is a mitogen-activated protein kinase (MAP) kinase kinase, which can initiate a mitogen-activated protein kinase (MAPK) cascade by phosphorylating the dual-specific MAP kinase kinases (MEK1/2), and in turn activate the extracellular signal-regulated kinases (ERK1/2). To study the function of c-Raf in teleost fish, a c-Raf cDNA sequence from orang...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Ancient pieces of amber found to contain dinosaur feathers riddled with louse-like insectsDinosaurs may have been fearsome and intimidating creatures that dominated the prehistoric earth – but it did not stop them having their feathers ruffled by parasites, researchers have found.Scientists have discovered ancient pieces of amber, dating from about 99m years ago, that contain dinosaur feathers riddled with louse-like insects. One of the feathers even shows signs of having been nibbled.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Dinosaurs Evolution Biology Fossils Science Zoology UK news Insects Animals World news Wildlife Environment Source Type: news
CONCLUSION: It has been noted that thiol/disulphide homeostasis in patients with autoimmune gastritis has changed in the way that ischemia modified albumin and disulphide increased whereas thiols decreased. Altered thiol/disulphide balance was also observed in patients with delayed gastric emptying. These results have suggested that the oxidative process is involved in patients with autoimmune gastritis. PMID: 31814373 [PubMed - as supplied by publisher]
Source: Turkish Journal of Medical Sciences - Category: General Medicine Tags: Turk J Med Sci Source Type: research
Source: Journal of Asthma and Allergy - Category: Allergy & Immunology Tags: Journal of Asthma and Allergy Source Type: research
The objective of this study is to identify single nucleotide polymorphisms (SNPs) of 12 candidate goat genes mainly related to the innate immune response associated with fecal egg counts (FECs) ofHaemonchus contortus in goat as an indicator of the level of parasite infection. Phenotypic data including FEC and blood traits were recorded in 189 native goats from China and 191 ones from Bangladesh, respectively. Bangladeshi goats had significantly (P
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
In this study, we conducted a molecular analysis of VP4, VP7, VP6 and NSP4 genes of RVAs detected using a commercial antigen ELISA in small ruminants with or without diarrhea in Turkey. Of the RVAs detected in sheep, one strain (Kutahya) was characterized as genotype G8P[1]-I2-E2. Two others (Ankara-1 and Ankara-2) were identified as NSP4 E2 and VP6 I2 genotypes, although they were untyped for the VP4 and VP7 genes. The RVAs from two goats were characterized as genotype G6P [1]-I2-E2. This is the first detection of in goats RVA genotypes G6P [1], which had previously only been found in cattle in Turkey, and of RVA in sheep...
Source: Archives of Virology - Category: Virology Source Type: research
Source: Risk Management and Healthcare Policy - Category: Health Management Tags: Risk Management and Healthcare Policy Source Type: research
Authors: Beltrán Rosel A, Sanjoaquín Conde I, Pérez Muñoz A, Alcalá PM, Marta CB, Irigoyen von Sierakowski A Abstract Strongyloides stercoralis is a parasitic nematode with a worldwide distribution. It can go from an asymptomatic infection to a life-threatening hyperinfection syndrome. Here, we report a case of intestinal obstruction due to S. stercoralis in a pregnant woman. This condition, as well as severe strongyloidiasis in pregnant women, is seldomly reported. In this case, Human Tlymphotropic Virus 1 (HTLV-1) coinfection was confirmed, a well-known risk factor for a more se...
Source: New Microbiologica - Category: Microbiology Tags: New Microbiol Source Type: research
Authors: Toscano JHB, Okino CH, Dos Santos IB, Giraldelo LA, von Haehling MB, Esteves SN, de Souza Chagas AC Abstract The immune response against Haemonchus contortus infections is primarily associated with the Th2 profile. However, the exact mechanisms associated with increased sheep resistance against this parasite remains poorly elucidated. The present study is aimed at evaluating mediators from the innate immune response in lambs of the Morada Nova Brazilian breed with contrasting H. contortus resistance phenotypes. Briefly, 287 lambs were characterized through fecal egg counts (FEC) and packed cell volume (PCV...
Source: Journal of Immunology Research - Category: Allergy & Immunology Tags: J Immunol Res Source Type: research
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