Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions

In this study, we evaluated a novel multiplex real-time PCR melting curve assay method for the simultaneous detection of 9 STD pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum, and herpes simplex virus.Methods: The analytical performance of the method, including its limit of detection (LOD), specificity, repeatability, and effect on different DNA extraction kits were evaluated. Additionally, we obtained 1,328 clinical specimens from 3 hospitals to detect the 9 STD pathogens using multiplex real-time PCR melting curve and Sanger sequencing, to evaluate the sensitivity, specificity, and consistency of the assay method.Results: The results showed that the analytical sensitivity of the novel multiplex real-time PCR melting curve assay is very excellent, with LOD of DNA corresponding to
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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CONCLUSIONS: An improved coordinated individual plan (CIP) process emerged, based on the active participation of users and caregivers. This process is a familiar and transparent process for users and caregivers, reflecting the needs and preferences of users at all stages. It requires careful preparation and collaboration with the users, as well as caregiver flexibility. PMID: 32940581 [PubMed - in process]
Source: International Journal of Qualitative Studies on Health and Well-being - Category: International Medicine & Public Health Tags: Int J Qual Stud Health Well-being Source Type: research
CONCLUSIONS: The AQUEOUS study assessed for the first time the unmet needs of young PAPS patients, enabling the development of a future "youth-focused" strategy to reduce disease burden. PMID: 32940209 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Rheumatology - Category: Rheumatology Tags: Clin Exp Rheumatol Source Type: research
Conclusions: The results indicate that fall risk measures within the ICF domains—body, structure, and function (dynamic gait stability and hip extensor strength) and activity limitation (TUG)—could provide a sensitive laboratory-induced slip-fall prediction model in PwCS. Video Abstract available for more insights from the authors (see the Video, Supplemental Digital Content 1, available at: http://links.lww.com/JNPT/A323).
Source: Journal of Neurologic Physical Therapy - Category: Physiotherapy Tags: Research Articles Source Type: research
Publication date: December 2020Source: Journal of Transport &Health, Volume 19Author(s): Abigail L. Cochran
Source: Journal of Transport and Health - Category: Occupational Health Source Type: research
Authors: Rosenberg CR, Abazari A, Chou TY, Weissbart SB Abstract A retrospective chart review examined adult patients with herpes simplex (HSV) and zoster (HZO) keratitis at a single institution. Patients who suffered a poor outcome (defined as visually significant corneal scarring, neurotrophic keratitis, secondary glaucoma, or requiring corneal surgery) were identified and each outcome type was analyzed in relation to age, sex, diabetes mellitus, immunosuppression, and a prior history of ≥2 ocular procedures. Advanced age, diabetes mellitus, and a prior history of ≥2 ocular procedures may be risk factors fo...
Source: Ocular Immunology and Inflammation - Category: Allergy & Immunology Tags: Ocul Immunol Inflamm Source Type: research
Publication date: Available online 17 September 2020Source: Pregnancy HypertensionAuthor(s): Yunha Noh, Seung-Ah Choe, Ju-Young Shin
Source: Pregnancy Hypertension: An International Journal of Womens Cardiovascular Health - Category: OBGYN Source Type: research
Publication date: Available online 17 September 2020Source: Pregnancy HypertensionAuthor(s): David Ramiro-Cortijo, María de la Calle, Pilar Rodriguez-Rodriguez, Sophida Phuthong, Ángel L. López de Pablo, Maria A. Martín-Cabrejas, Silvia M. Arribas
Source: Pregnancy Hypertension: An International Journal of Womens Cardiovascular Health - Category: OBGYN Source Type: research
Publication date: Available online 17 September 2020Source: Pregnancy HypertensionAuthor(s): Ljiljana Mirkovic, Ivan Tulic, Sanja Stankovic, Ivan Soldatovic
Source: Pregnancy Hypertension: An International Journal of Womens Cardiovascular Health - Category: OBGYN Source Type: research
No abstract available
Source: Postgraduate Obstetrics and Gynecology - Category: OBGYN Tags: Article Source Type: research
This article describes the legal framework for service animals and ESAs, as well as the differences between them. We summarize information about the Americans with Disabilities Act, the Fair Housing Act, the Air Carrier Access Act, and other laws governing an individual's right to be accompanied by a support animal. We also summarize the clinical research on ESAs and argue that, although there are few studies on the clinical effectiveness of ESAs, a broader body of research indicates that animals may have positive clinical effects on medical and mental illness. Finally, we suggest there is a need for further research and p...
Source: Journal of the American Academy of Psychiatry and the Law - Category: Medical Law Tags: J Am Acad Psychiatry Law Source Type: research
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