Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions

In this study, we evaluated a novel multiplex real-time PCR melting curve assay method for the simultaneous detection of 9 STD pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum, and herpes simplex virus.Methods: The analytical performance of the method, including its limit of detection (LOD), specificity, repeatability, and effect on different DNA extraction kits were evaluated. Additionally, we obtained 1,328 clinical specimens from 3 hospitals to detect the 9 STD pathogens using multiplex real-time PCR melting curve and Sanger sequencing, to evaluate the sensitivity, specificity, and consistency of the assay method.Results: The results showed that the analytical sensitivity of the novel multiplex real-time PCR melting curve assay is very excellent, with LOD of DNA corresponding to
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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Authors: Di Carlo M, Muto P, Benfaremo D, Luchetti MM, Atzeni F, Salaffi F Abstract OBJECTIVE: To evaluate the prevalence and factors associated with the neuropathic pain features in a cohort of patients with psoriatic arthritis (PsA). METHODS: A cross-sectional evaluation was conducted in consecutive patients suffering from PsA with prevalent peripheral joint involvement, referring to three rheumatological centers. For each patient a comprehensive assessment of disease activity, physical function, and disease impact was carried out. The presence of comorbid fibromyalgia syndrome (FMS) was evaluated. Acute phas...
Source: Journal of Rheumatology - Category: Rheumatology Tags: J Rheumatol Source Type: research
Publication date: Available online 4 December 2019Source: NeuroImageAuthor(s): Dennis M. Hedderich, Josef G. Bäuml, Aurore Menegaux, Mihai Avram, Marcel Daamen, Claus Zimmer, Peter Bartmann, Lukas Scheef, Henning Boecker, Dieter Wolke, Christian Gaser, Christian SorgAbstractPremature birth bears an increased risk for aberrant brain development concerning its structure and function. Cortical complexity (CC) expresses the fractal dimension of the brain surface and changes during neurodevelopment. We hypothesized that CC is altered after premature birth and associated with long-term cognitive development.One-hundred-and-...
Source: NeuroImage - Category: Neuroscience Source Type: research
Conclusionsthe present study offers additional support to the usefulness of longitudinal S100B and drug level monitoring in depressed pregnant women and in the early detection of cases at risk for short-term neurological abnormalities. Results open the way at further investigations correlating antidepressant drugs and neurobiomarkers in the maternal bloodstream.
Source: Clinica Chimica Acta - Category: Laboratory Medicine Source Type: research
CONCLUSIONS: Traumatic brain injury-specific education and frequent assessments improved compliance between TBI-2012 and TBI-2017, resulting in a higher percentage in overall survivors in the latter group. PMID: 31789987 [PubMed - in process]
Source: Dimensions in Critical Care Nursing - Category: Nursing Tags: Dimens Crit Care Nurs Source Type: research
This study describes the clinical and radiographic outcomes of patients treated with expandable interbody spacers for MIS LLIF.MethodsA retrospective, single surgeon, clinical and radiographic study was performed on 37 consecutive patients who underwent MIS LLIF at 1–2 contiguous level(s) using expandable spacers. Radiographic and clinical functional outcomes were collected and compared at preoperative and postoperative time points up to 12 months. Parametric and nonparametric tests were used when appropriate with p value
Source: Interdisciplinary Neurosurgery - Category: Neurosurgery Source Type: research
Publication date: Available online 3 December 2019Source: Small Ruminant ResearchAuthor(s): Santiago Luzardo, Guillermo De Souza, Graciela Quintans, Georgget Banchero
Source: Small Ruminant Research - Category: Zoology Source Type: research
Publication date: Available online 3 December 2019Source: Small Ruminant ResearchAuthor(s): Ja’far Al-Khaza’leh, Bekele Megersa, Belal ObeidatAbstractA single-visit survey was conducted from June to October, 2017 to estimate the kid and lamb mortalities, and identify major causes and risk factors in Karak governorate of Jordan. Semi-structured questionnaires were administered to 120 small ruminant farmers in addition to focus group discussions. Results showed that diseases, feed shortage, poor mothering, predators and insufficient milk provision were perceived to be the most important causes of mortality in kid...
Source: Small Ruminant Research - Category: Zoology Source Type: research
In conclusion, the 4 protocols investigated were effective in synchronizing oestrus with similar response to synchronization of oestrus and fertility among treatment groups. Of the 4 protocols, the Ramlong protocol offers the benefit of being less costly because of reduced hormonal use in addition to the adequate fertility obtained.
Source: Small Ruminant Research - Category: Zoology Source Type: research
Publication date: Available online 4 December 2019Source: Genomics, Proteomics &BioinformaticsAuthor(s): Guan Ning Lin, Sijia Guo, Xian Tan, Weidi Wang, Wei Qian, Weichen Song, Jingru Wang, Shunying Yu, Zhen Wang, Donghong Cui, Han WangAbstractDe novo variants (DNVs) are one of the most significant contributors to severe early-onset genetic disorders such as autism spectrum disorder, intellectual disability, and other developmental and neuropsychiatric (DNP) disorders. Presently, a plethora of DNVs have been identified using next-generation sequencing, and many efforts have been made to understand their impact at the g...
Source: Genomics, Proteomics and Bioinformatics - Category: Bioinformatics Source Type: research
Authors: Choi M, Butler E, Clarke A, Girard LP, Gibson P, Skeith L Abstract Introduction: Systemic lupus erythematosus (SLE)-related thrombocytopenia during pregnancy and the postpartum period have been associated with adverse pregnancy outcomes and perinatal complications. In this case report, we present two SLE patients with thrombocytopenia emergencies secondary to HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome and thrombotic thrombocytopenic purpura (TTP).Areas covered: The first case involved a 26-year-old woman, G1P0 at 26 weeks gestation (GA), with high-titre antiphospholipid antibodie...
Source: Expert Review of Clinical Immunology - Category: Allergy & Immunology Tags: Expert Rev Clin Immunol Source Type: research
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