Cutinases as Stereoselective Catalysts: Specific Activity and Enantioselectivity of Cutinases and Lipases for Menthol and its Analogs

Publication date: Available online 12 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): An Su, Serpil Kiokekli, Mariam Naviwala, Abhijit N.Shirke, Ioannis V. Pavlidis, Richard A. GrossAbstractThe specific activity and enantioselectivity of immobilized cutinases from Aspergillus oryzae (AoC) and Humicola insolens (HiC) were compared with those of lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML) and Lipase B from Candida antarctica (CALB) for menthol and its analogs that include isopulegol, trans-2-tert-butylhexanol (2TBC), and dihydrocarveol (DHC). Common features of these alcohols are two bulky substituents: a cyclohexyl ring and an alkyl substituent. Dissimilarities are that the alkyl group reside at different positions or have dissimilar structures. The aim was to develop an understanding at a molecular level of similarities and differences in the catalytic behavior of the selected cutinases and lipases as a function of substrate structural elements. The experimental results reflect the (-)-enantioselectivity for AoC, HiC, TLL, and RML, while CALB is only active on DHC with (+)-enantioselectivity. In most cases, AoC has the highest activity while HiC is significantly more active than other enzymes on 2TBC. The E values of AoC, HiC, TLL, and RML for menthol are 27.8, 16.5, 155, and 125, respectively. HiC has a higher activity (>10-fold) on (-)-2TBC than AoC while they exhibit similar activities on menthol. Docking results reveal that the bu...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research

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Publication date: February 2020Source: Phytochemistry Letters, Volume 35Author(s): Gamal A. Mohamed, Sabrin R.M. Ibrahim, Hani Z. AsfourAbstractTwo new diaryl ether derivatives [aspergillethers A (4) and B (5)] and five known metabolites [(22E,24R)-stigmasta-5,7,22-trien-3-β-ol (1), stigmasta-4,6,8(14),22-tetraen-3-one (2), orcinol (3), and butyrolactones I (6) and VI (7)] were separated from the endophytic fungus Aspergillus versicolor (Aspergillaceae) isolated from the roots of Pulicaria crispa Forssk (Asteraceae) growing in Saudi Arabia. The structures of these compounds were determined by various spectral techniqu...
Source: Phytochemistry Letters - Category: Chemistry Source Type: research
There are only few drugs available to treat fungal infections, and the lack of new antifungals, along with the emergence of drug-resistant strains, results in millions of deaths/year. An unconventional approach to fight microbial infection is to exploit nutritional vulnerabilities of microorganism metabolism. The metal gallium can disrupt iron metabolism in bacteria and cancer cells, but it has not been tested against fungal pathogens such as Aspergillus and Candida. Here, we investigate in vitro activity of gallium nitrate III [Ga(NO3)3] against these human pathogens, to reveal the gallium mechanism of action and understa...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Publication date: Available online 2 December 2019Source: Journal of Molecular StructureAuthor(s): R. Rama, S. MeenakshiAbstractA novel Aliquat 336 based functionalized ionic liquid, methyltrioctylammonium naphthylacetate ([A336]+[NAA]-) have been synthesized and characterized using IR, UV, 1H NMR and Raman spectroscopy techniques. The prepared IL was tested for antimicrobial properties and chromium (VI) extraction. Anti-bacterial test was carried out towards five types of bacteria namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, Bacillus subtilis and Salmonella typhi. The antifungal test was done ou...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Abstract Condensation of rhodanine (1) with pyrazol-3(2H)-one derivatives (2a-f) gave 5-substituted-2-thioxo-1,3-thiazolidin-4-one derivatives (3a-f). Reaction of compound (1) with 2-arylmethylidene-malononitrile (4a-d) yielded the unexpected derivatives (5a-d). The latter compounds were subjected to cyclization reactions with malononitrile under different basic conditions, hydroxylamine hydrochloride and/or thiourea to furnish the fused thiazole derivatives (6a-d) and (8-10a-d). Coupling of (1) with diazotized aromatic amines (11a-c) in pyridine afforded the arylhydrazones (12a-c). Fusion of latter compounds with...
Source: Chemical and Pharmaceutical Bulletin - Category: Drugs & Pharmacology Authors: Tags: Chem Pharm Bull (Tokyo) Source Type: research
Publication date: Available online 30 November 2019Source: Food Research InternationalAuthor(s): Guiqiang He, Jun Huang, Chongde Wu, Yao Jin, Rongqing ZhouAbstractTraditional spontaneous fermentation in microecosystem with microbial successions and environmental variables results in inconsistent quality of fermented foods. We therefore propose the directional bioturbation for microbiota regulation and metabolites production in food fermentation. Here, we revealed the bioturbation effect of fortified Daqu on microbial community based on taxonomic composition, co-occurrence network, and metabolic potential, using Chinese str...
Source: Food Research International - Category: Food Science Source Type: research
Publication date: Available online 28 November 2019Source: Microbiological ResearchAuthor(s): Sohini Chatterjee, Ranjan Ghosh, Narayan Chandra MandalAbstractFungal resistance against frequently used antifungal medicines used for invasive candidiasis and other fungal infections is directing scientist for searching and developing novel antifungal drugs. An endophytic fungal strain Alternaria tenuissima OE7 has been isolated from leaves of Ocimum tenuiflorum L. which showed antifungal activity against numbers of human pathogenic fungi including Trichophyton rubrum, Microsporum gypseum, Aspergillus parasiticus, A. flavus, A. f...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research
Publication date: Available online 22 November 2019Source: Carbohydrate PolymersAuthor(s): Joslaine Jacumazo, Mariana M. de Carvalho, Gabriela Pereira Parchen, Izabela M.F. Campos, Meira J. Ballesteros Garcia, Tatiane Brugnari, Giselle Maria Maciel, Francisco A. Marques, Rilton A. de FreitasAbstractThis work describes the production of polysaccharide multilayer capsules to control the release of Eugenol (Eug) and enabling its use as an antimicrobial agent. For this propose, oil-in-water nanoemulsions of sodium dodecyl sulfate were coated with alternating depositions of chitosan and carboxymethylcellulose using the Layer-by...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Conclusions: Specific serum IgGs may point towards exposure source in HP patients.G1207 Roche, MZ ČR RVO- TN 00064190
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: ILD/DPLD of known origin Source Type: research
Background: Studies investigating sensitization caused by various mycotic allergens and Aspergillus fumigatus components are lacking. We aimed to measure the prevalence of sensitization to fungal allergens and Aspergillus fumigatus components among patients in southern China.Methods: 4033 patients with respiratory diseases were enrolled to analyze the prevalence of Aspergillus fumigatus; 195 adult patients were further examined for sIgE against Aspergillus fumigatus, Penicillium chrysogenum, Cladosporium herbarum, Mucor racemosus, Candida albicans, Alternaria alternata, and Helminthosporium halodes; and 18 ABPA and 33 asth...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Allergy and immunology Source Type: research
Conclusion: A more pronounced quantitative content and a more diverse microbial landscape leads to a more severe course of the disease asthma combined with COPD, especially of the bronchitis type.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Allergy and immunology Source Type: research
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