Small molecule fluorescent probe: illumining and monitoring foreign proteins based on high fidelity imaging in living cells
Publication date: Available online 12 November 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Yingcui Bu, Huihui Zhang, Zhiheng Li, Aidong Wang, Junjun Wang, Xiaojiao Zhu, Jie Zhang, Weiju Zhu, Guangmei Chen, Hongping ZhouAbstractTraditional biotechnology, taking advantage of fluorescent protein (FP) to investigate the foreign protein, was difficult to visualize and image accurately in living cells. Herein, bovine serum albumin (BSA) was illumined in virtue of the prepared probe 1, which could monitor the foreign protein of the intracellular recognition, transportation and metabolism under confocal microscopy. Verified by 1H NMR titration experiment and molecular docking simulation, the probes bonded to BSA mainly through hydrogen bond and suitable hydrophobicity, realizing the visualization and high-fidelity imaging of the foreign protein in living cells. The results provided the beneficial approach and methodology for further understanding protein metabolism.Graphical abstractFluorescence probe 1 with high specificity towards foreign protein BSA delivered “turn on” fluorescence. Furthermore, the probe 1 accomplished the visualization of the foreign protein BSA ingested into cells under the confocal laser scanning microscopy (CLSM), which exhibited targeting ability towards subcellular organelles related with proteins such as endoplasmic reticulum, lipids droplets, and lysosome.
Despite existing evidence that gait disorders are a common consequence of severe traumatic brain injury (sTBI), the literature describing gait instability in sTBI survivors is scant. Thus, the present study aims at quantifying gait patterns in sTBI through...
CONCLUSION: The 3D canine jaw fractures models, which reproduced natural canine jaw fractures, and their respective radiographic images, are a possible source of educational material for the teaching of veterinary medicine. PMID: 31826098 [PubMed - in process]
ConclusionIn Revolution CT, beam hardening artifacts were reduced in reduced-iodine VMI at lower energy levels compared to Discovery CT, contributing to better image quality.
ConclusionsPregnancy after liver transplantation has a favorable outcome with a multidisciplinary team approach. There is a physiological reduction of tacrolimus trough levels during pregnancy for which dose augmentation is not usually required.
Publication date: December 2019Source: British Journal of Oral and Maxillofacial Surgery, Volume 57, Issue 10Author(s): Sukhpreet Dubb, Stephanie Agar, Janhvi Shah, Paayal Shah, Tahir Mirza
Conclusions: Herniation of TMJ into the EAC is a rare condition, but can be encountered in the clinic at any time. This literature review could be helpful in the diagnosis and treatment of TMJ herniation into the EAC.
Conclusion: The reduction of tinnitus in the current ABI subject may be attributable to partial peripheral reafferentation-induced deactivation of the parahippocampus-based tinnitus generator as well as the salience network. Further validation is required by the use of a follow-up study with a larger number of subjects.
Conclusions: Poor cavity obliteration, a high rate of revision surgery and difficult differentiation between recurrent cholesteatoma and granulation tissue in CT scan was observed.
Conclusion: Results for quantitative evaluation were similar in both the sequences. Gadolinium injection can be avoided only in surveillance studies.
CONCLUSION: Flexible videoureterorrenoscopy and Holmium laser for the management of intrarenal stenosis are highly effective and safe with the advantages of less perioperative complications, less invasiveness and being an outpatient procedure compared to percutaneous surgery. PMID: 31823855 [PubMed - in process]