Antileishmanial effect of rapamycin as an alternative approach to control Leishmania tropica infection

Publication date: Available online 10 November 2019Source: Veterinary ParasitologyAuthor(s): Fatemeh Khadir, Tahereh Taheri, Sima Habibzadeh, Farnaz Zahedifard, Elham Gholami, Maryam Heidari-Kharaji, Ahmad Oryan, Sima RafatiAbstractCutaneous leishmaniosis (CL) is a parasitic disease in animals and human with no satisfactory treatments and vaccination. Rapamycin is a potent inhibitor of mammalian target of rapamycin (mTOR) with various applications. Here, the effect of rapamycin alone or in combination with two other drugs, namely amphotericin B (AmB) and glucantime, was investigated against Leishmania tropica infection. In vitro viability and electron microscopy evaluation of the parasites showed detrimental changes in their appearance and viability. Treatment with clinically relevant dose of rapamycin (10.2 µg/dose) is able to control the parasite load in BALB/c mice infected with L. tropica. Furthermore, the cytokine profiles showed significant polarization towards Th1 immune response. Surprisingly, combination therapy with either AmB or glucantime was not efficient. Rapamycin is showed an effective alternative therapy against leishmaniosis caused by L. tropica.Graphical abstract
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research

Related Links:

This study assessed the potential role of the Sub-Saharan rodents Arvicanthis neumanni, A. niloticus and Mastomys natalensis as hosts of L. donovani and L. sp. from Ghana, based on experimental infections of animals and xenodiagnoses. The distribution and load of parasites were determined post mortem using qPCR from the blood, skin and viscera samples. The attractiveness of Arvicanthis and Mastomys to Phlebotomus orientalis was tested by pair-wise comparisons. None of the animals inoculated with L. donovani were infectious to P. orientalis females, although, in some animals, parasites were detected by PCR even 30 weeks pos...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Conclusion: Leishmania major is responsible for the most common CL in southern Iran. In addition, in two patients with L. major and L. tropica, mix infection with Crithidia was confirmed. The potential role of Crithidia as the main factor for CL and the probability of this parasite to have synergistic effects on Leishmania, as a hypothesis, requires more comprehensive researches on the ambiguity of this protozoon. PMID: 31803781 [PubMed]
Source: Iranian Journal of Arthropod-Borne Diseases - Category: Infectious Diseases Tags: J Arthropod Borne Dis Source Type: research
Canine leishmaniosis (CanL) caused by Leishmania infantum can have several dermatological manifestations. The type of immune response elicited against the parasite appears to be at the basis for such clinical var...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Abstract A series of bis-naphthoquinone derivatives prepared by condensation of aryl aldehydes with lawsone was tested for anti-parasitic activities against Toxoplasma gondii and Trypanosoma brucei parasites. Monofluorophenyl derivative 1a , 3,4-difluorophenyl analog 1c and furyl compound 1l exhibited significant activity against T. gondii cells and appear to be new promising drug candidates against this parasite. The 3,4,5-trifluorophenyl derivative 1g and the isovanillyl derivative 1j displayed selective activity against Leishmania major amastigotes. PMID: 31804031 [PubMed - as supplied by publisher]
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research
Leishmaniases are widespread neglected diseases with an incidence of 1.6 million new cases and 40 thousand deaths per year. Leishmania parasites may show distinct, species-specific patterns of virulence that lead to different clinical manifestations. It is well known that successive in vitro passages (SIVP) lead to the attenuation of virulence, but neither the metabolism nor the pathways involved in these processes are well understood. Herein, promastigotes of a virulent L. amazonensis strain recently isolated from mice was compared to SIVP derived and attenuated promastigotes, submitted to 10, 40, and 60 axenic passages a...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
This study indicates the potential of the essential oil of O. onites and its constituents in the treatment of protozoal infections.
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
AbstractCutaneous leishmaniasis (CL) is a neglected disease that promotes destructive lesions. Difficulties in treatment are related to accessibility of drugs, resistance and toxicity. Antimicrobial photodynamic therapy (APDT) has been emerging as a promising treatment for CL. In this work, we evaluated methylene blue (MB) ‐mediated APDT (MB‐APDT) onLeishmania amazonensis in vitro andin vivo by bioluminescence technique.In vitro, MB ‐APDT was performed using a red LED (λ= 660±11 nm, 100 mW/cm2) and MB (100 µM) at different light doses.In vivo, mice were infected and 4 weeks later, randomly divided...
Source: Photochemistry and Photobiology - Category: Science Authors: Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research
This study reported the function of the unknown proteins ofL. donovani which is imperative information for this parasite. They could be an important and promising target for new drug discovery vaccine candidate development. Further characterization and in-depth study of these unknown proteins may open a gate for successful therapy of fatal VL.
Source: Network Modeling Analysis in Health Informatics and Bioinformatics - Category: Bioinformatics Source Type: research
We present a CVL case involving a 2-year-old male dog with a history of lack of appetite, general weakness, and progressive loss of weight. A diagnosis of CVL was obtained using the direct parasitological examination in spleen and bone marrow samples stained with Giemsa. The infecting Leishmania species was identified as L. infantum by PCR-restriction fragment length polymorphism amplifying the Hsp70 gene from bone marrow and spleen samples. The patient responded favorably to treatment with intramuscular meglumine antimoniate at a dose of 100 mg/kg daily for 8 weeks and oral allopurinol at a dose of 10 mg/kg every 12 hours...
Source: Am J Trop Med Hyg - Category: Infectious Diseases Authors: Tags: Am J Trop Med Hyg Source Type: research
by Sarah Hendrickx, Lieselotte Van Bockstal, Guy Caljon, Louis Maes Monitoring the drug susceptibility ofLeishmania isolates still largely relies on standardin vitro cell-based susceptibility assays using (patient-isolated) promastigotes for infection. Although this assay is widely used, no fully standardized/harmonized protocol is yet available hence resulting in the application of a wide variety of host cells (primary cells and cell lines), different drug exposure times, detection methods and endpoint criteria. Advocacy for standardization to decrease inter-laboratory variation and improve interpretation of results has ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
More News: Abelcet | Alternative and Complementary Therapies | HIV-Leishmania Co-infection | Parasitic Diseases | Parasitology | Vaccines | Veterinary Research | Veterinary Vaccinations