Salmonella Serotyping; Comparison of the Traditional Method to a Microarray-Based Method and an in silico Platform Using Whole Genome Sequencing Data

Salmonella is one of the most common causes of food-borne diseases worldwide. While Salmonella molecular subtyping by Whole Genome Sequencing (WGS) is increasingly used for outbreak and source tracking investigations, serotyping remains as a first-line characterization of Salmonella isolates. The traditional phenotypic method for serotyping is logistically challenging, as it requires the use of more than 150 specific antisera and well trained personnel to interpret the results. Consequently, it is not a routine method for the majority of laboratories. Several rapid molecular methods targeting O and H loci or surrogate genomic markers have been developed as alternative solutions. With the expansion of WGS, in silico Salmonella serotype prediction using WGS data is available. Here, we compared a microarray method using molecular markers, the Check and Trace Salmonella assay (CTS) and a WGS-based serotype prediction tool that targets molecular determinants of serotype (SeqSero) to the traditional phenotypic method using 100 strains representing 45 common and uncommon serotypes. Compared to the traditional method, the CTS assay correctly serotyped 97% of the strains, four strains gave a double serotype prediction. Among the inconclusive data, one strain was not predicted and two strains were incorrectly identified. SeqSero was evaluated with two versions (SeqSero 1 and the alpha test version of SeqSero 2). The correct antigenic formula was predicted by SeqSero 1 for 96 and 95% of...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Despite an outbreak of vegetable-associated Salmonella poisoning linked to products sold at Massachusetts restaurants that has affected 90 Massachusetts residents and resulted in three deaths, the Massachusetts Department of Public Health is refusing to release any information on the restaurants at which the patients ate or the exact products they consumed. The Department has revealed only that the outbreak is associated with " vegetable products " and that some of the patients reported having eaten vegetables at restaurants in the Commonwealth.The Department of Public Health is not even telling the restaurant ow...
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Recent clusters of an uncommon strain of Salmonella traced to raw turkey products suggest that this strain may be widespread in the turkey industry.Morbidity &Mortality Weekly Report
Source: Medscape Today Headlines - Category: Consumer Health News Tags: Public Health & Prevention Journal Article Source Type: news
Publication date: Available online 4 December 2019Source: Food Research InternationalAuthor(s): ZAHRA H. MOHAMMAD, ELSA A. MURANO, ROSANA G. MOREIRA, ALEJANDRO CASTILLOAbstractSince two outbreaks of salmonellosis were linked to the consumption of almonds in 2001 and 2004, the study of pathogen inactivation kinetics in almonds has been encouraged, often by conducting inoculated challenge studies. The inoculation method could affect the results of such challenge studies, because of the possible increase of moisture on the almonds resulting from a wet inoculation procedure, which may result in a potential overestimation of th...
Source: Food Research International - Category: Food Science Source Type: research
Publication date: April 2020Source: Food Control, Volume 110Author(s): Haijing Hu, Kun Jia, Huhu Wang, Xinglian Xu, Guanghong Zhou, Shuwen HeAbstractSalmonella is the leading cause of infectious disease outbreaks associated with meat products, residual meat particles on food-contact surfaces can influence the biofilm formation of microorganisms. The transcriptional landscape and expression of novel small non-coding RNAs (sRNAs) were investigated during the biofilm formation of S. Enteritidis exposed to meat thawing loss broth (MTLB) in this study. The genes of csgB, adrA and fimH were differentially expressed. Notably, a t...
Source: Food Control - Category: Food Science Source Type: research
Publication date: Available online 29 November 2019Source: Food Research InternationalAuthor(s): Stéfani T.A. Dantas, Carlos H. Camargo, Monique R. Tiba-Casas, Ricardo C. Vivian, José P.A.N. Pinto, José C.F. Pantoja, Rodrigo T. Hernandes, Ary Fernandes Júnior, Vera L.M. RallAbstractSalmonella spp. is responsible for severe foodborne disease, and is one of the main agents involved in foodborne outbreaks worldwide. Contamination occurs mainly as a result of poultry and egg consumption since they can carry some serotypes pathogenic to humans. The aim of the study was to evaluate the persistence and...
Source: Food Research International - Category: Food Science Source Type: research
In this study, different combinations of bacteriophages (phages) and cinnamaldehyde (CNMA) were incorporated on sodium alginate emulsion-based films to impart them with antimicrobial activity towards S. Enteritidis and E. coli. Films were prepared by casting and they were characterized in terms of CNMA and/or phages loading, thickness, moisture content, water vapor permeability (WVP), swelling index (SW), chemical interactions by FTIR, surface morphology by SEM and antimicrobial performance. Results showed that phages incorporation was not compromised by CNMA as evidenced by their viability inside the films. Increasing CNM...
Source: Food Research International - Category: Food Science Source Type: research
Publication date: Available online 21 November 2019Source: Food Research InternationalAuthor(s): Christina Sakarikou, Dimitra Kostoglou, Manuel Simões, Efstathios GiaourisAbstractSalmonella is one of the most frequent causes of foodborne outbreaks throughout the world. In the last years, the resistance of this and other pathogenic bacteria to antimicrobials has become a prime concern towards their successful control. In addition, the tolerance and virulence of pathogenic bacteria, such as Salmonella, are commonly related to their ability to form biofilms, which are sessile structures encountered on various surfaces ...
Source: Food Research International - Category: Food Science Source Type: research
In a new report, researchers from the U.S. Centers for Disease Control and Prevention detail the tracking of a recent multistate outbreak of salmonella infections linked to raw turkey products.
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PMID: 31751325 [PubMed - in process]
Source: MMWR Morb Mortal Wkl... - Category: Epidemiology Authors: Tags: MMWR Morb Mortal Wkly Rep Source Type: research
In the United States, the consumption of fresh fruits and vegetables has increased during recent years as consumers seek to make healthier lifestyle choices. However, the number of outbreaks associated with fresh produce that involve cases in more than one state (multistate) has increased concomitantly. As the distance along the farm-to-fork continuum has lengthened over time, there are also more opportunities for fresh produce contamination with bacterial pathogens before it reaches the consumer. This review provides an overview of the three bacterial pathogens (i.e., pathogenic Escherichia coli, Listeria monocytogenes, a...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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