GSE122362 Systems biology of S.aureus adaptation under Ser/Thr kinase wild type and mutations of kinase (pknB) or phosphatase (stp)

Contributors : Liang Chunguang ; Rios-Miguel Ana ; Jarick Marcel ; Priya Neurgaonkar ; Patrice Francois ; Jacques Schrenzel ; Knut Ohlsen ; Dandekar ThomasSeries Type : Expression profiling by arrayOrganism : Staphylococcus aureus ; Staphylococcus aureus subsp. aureus str. NewmanDifferent transcriptome, metabolome, and phosphoproteome studies have already indicated a regulatory role of Ser/Thr phosphorylation in the central metabolism of S. aureus. However, it remains still unknown how this exactly tunes its metabolism. For that reason, Extreme Pathway Analysis was used to understand metabolic flux regulation by Ser/Thr phosphorylation.YANAsquare software and YANAvergence routine were chosen to calculate pathway activities of WT, ΔpknB, Δstp, and double mutant S. aureus NewmanHG strains.Gene expression data of S. aureus NewmanHG were collected taking the wild type and mutations of kinase (pknB), phosphatase (stp) or both (pknB/stp) phenotypes. The resulting flux changes were modelled with YANAvergence taking the different gene expression data and an established genome scale model of S. aureus metabolic modes as basis. The fluxes of the different strains were next calculated, taking the gene expression data into account.A concerted metabolic change of fluxes was observed. In particular the pathways for peptidoglycan, nucleotide, and aromatic amino acid synthesis as well as catabolism involving aspartate transaminase (GOT) changed significantly compared to the wild ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Staphylococcus aureus Staphylococcus aureus subsp. aureus str. Newman Source Type: research

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