ASAP Molecular Bases of DNA Packaging in Bacteria Revealed by All-Atom Molecular Dynamics Simulations: The Case of Histone-Like Proteins in Borrelia burgdorferi

The Journal of Physical Chemistry LettersDOI: 10.1021/acs.jpclett.9b02978
Source: The Journal of Physical Chemistry Letters - Category: Chemistry Authors: Source Type: research

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Publication date: Available online 6 December 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Hein Sprong, Sander Moonen, Sipke E. van Wieren, Tim R. HofmeesterAbstractCattle grazing has been suggested to reduce the risk for Lyme borreliosis by decreasing the density of questing Ixodes ricinus infected with Borrelia burgdorferi sensu lato. We tested the hypotheses that cattle grazing used in woodland management decreases the density of questing I. ricinus, and that it decreases the nympal infection prevalence of B. burgdorferi sensu lato. We further expected the nympal infection prevalence of tick-borne pathogens that ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
(CNN/CBS) — The climate crisis is making us more vulnerable to ticks in an unprecedented and growing threat to public health, according to a new report. Bacterial and protozoan tick-borne diseases doubled in the United States between 2004 and 2016, the report notes, and in 2017, more than 90% of the 60,000 vector-borne diseases in the United States were linked to these particular bloodsucking bugs. The warmer temperatures brought by the climate crisis, in addition to ecological changes and reforestation, have extended the bugs’ range into regions that had not seen certain types of ticks for many decades, if eve...
Source: WBZ-TV - Breaking News, Weather and Sports for Boston, Worcester and New Hampshire - Category: Consumer Health News Authors: Tags: Boston News Health Syndicated CBSN Boston Lone Star Tick ticks Source Type: news
Abstract Borrelia species are vector-borne parasitic bacteria with unusual, highly fragmented genomes that include a linear chromosome and linear as well as circular plasmids that differ numerically between and within various species. Strain CA690T, which was cultivated from a questing Ixodes spinipalpis nymph in the San Francisco Bay area, CA, was determined to be genetically distinct from all other described species belonging to the Borrelia burgdorferi sensu lato complex. The genome, including plasmids, was assembled using a hybrid assembly of short Illumina reads and long reads obtained via Oxford Nanopore Tec...
Source: International Journal of Systematic and Evolutionary Microbiology - Category: Microbiology Authors: Tags: Int J Syst Evol Microbiol Source Type: research
In this study, we determined the crystal structures of the key outer surface protein BBE31 from B. burgdorferi and its orthologous protein BSE31 (BSPA14S_RS05060 gene product) from B. spielmanii. BBE31 is known to be important for the transfer of B. burgdorferi from the gut to the hemolymph in the tick after a tick bite. While BBE31 exerts its function by interacting with the Ixodes scapularis tick gut protein TRE31, structural and mass spectrometry data revealed that BBE31 has a glutathione (GSH) covalently attached to Cys142 suggesting that the protein may have acquired some additional functions in contrast to its orthol...
Source: Biochimica et Biophysica Acta (BBA) General Subjects - Category: Biochemistry Source Type: research
We report a case of suspected Lyme neuroborreliosis (LNB) where the patient's sole symptom was chronic, intermittent episodes of unilateral ear pain. This case was unusual because this patient did not show any other neurological or extraneurological symptoms of Lyme disease. LNB is a manifestation of infection by Borrelia burgdorferi spirochetes that can manifest in a number of different clinical presentations depending on the nervous system dissemination of the bacteria. Most commonly, these neurological findings present as peripheral nerve radiculopathies, but rarer involvement of the central nervous system (CNS) can...
Source: Journal of Global Infectious Diseases - Category: Infectious Diseases Authors: Source Type: research
In conclusion, here we describe the molecular mechanism utilized by B. mayonii to resists complement-mediated killing by capturing human immune regulators.
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
We present a case of a man with genital condyloma acuminatum with a positive T. pallidum IHC stain but negative T. pallidum serologies and no syphilitic symptoms. It has been shown that the T. pallidum antibody IHC can cross-react, staining other spirochetes, including Borrelia burgdorferi and the Brachyspira family of intestinal spirochetes. Because of the proximity of our patient's lesions to the anus, and the persistently negative T. pallidum serologies, we believe nontreponemal spirochetes colonized the condyloma, giving a false-positive T. pallidum IHC. This cross-reactivity is a potential diagnostic pitfall and is im...
Source: The American Journal of Dermatopathology - Category: Pathology Tags: Brief Report Source Type: research
Single multiplexed assays could replace the standard 2-tiered (STT) algorithm recommended for the laboratory diagnosis of Lyme disease if they perform with a specificity and a sensitivity superior or equal to those of the STT algorithm. We used human serum rigorously characterized to be sera from patients with acute- and convalescent-phase early Lyme disease, Lyme arthritis, and posttreatment Lyme disease syndrome, as well as the necessary controls (n = 241 samples), to select the best of 12 Borrelia burgdorferi proteins to improve our microfluidic assay (mChip-Ld). We then evaluated its serodiagnostic performance in compa...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research
Vector-Borne and Zoonotic Diseases, Ahead of Print.
Source: Vector-Borne and Zoonotic Diseases - Category: Infectious Diseases Authors: Source Type: research
Conclusions: Because serum serology can be negative at presentation, lumbar puncture is a valuable tool when diagnosing LB among children with facial palsy. Pleocytosis and increased protein and CXCL13 values in the CSF suggest LB as the cause of facial palsy.
Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Original Studies Source Type: research
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