Ultrasound evaluation of seminiferous tubules: a promising prognostic tool for men with nonobstructive azoospermia undergoing microsurgical testicular sperm extraction

Azoospermia, or the absence of spermatozoa in the semen, is present in up to 10% of men presenting with infertility. The majority of these men will have nonobstructive azoospermia (NOA), or impaired production of sperm. When present, testicular sperm can be used with in  vitro fertilization and intracytoplasmic sperm injection to achieve fertility. One of the most effective treatment options to obtain testicular sperm is microsurgical testicular sperm extraction (micro-TESE). Reported rates of successful sperm retrieval with micro-TESE range from 40% to 60%.
Source: Fertility and Sterility - Category: Reproduction Medicine Authors: Tags: Reflections Source Type: research

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Authors: Cui X, Wu X, Li Q, Jing X Abstract Congenital bilateral absence of the vas deferens (CBAVD) is predominantly caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. CBAVD accounts for 2‑6% of male infertility cases and up to 25% of cases of obstructive azoospermia. With the use of pre‑implantation genetic diagnosis, testicular or epididymal sperm aspiration, intracytoplasmic sperm injection and in vitro fertilization, patients affected by CBAVD are able to have children who do not carry CFTR gene mutations, thereby preventing disease. Therefore, genetic counseli...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
This article elaborates on the distribution and immune response characteristics of epididymis immune cells, the role of epididymis epithelial cells (EECs), and the epididymis defense against different pathogenic infections (such as uropathogenic Escherichia coli, Chlamydia trachomatis, and viruses to provide therapeutic approaches for epididymitis and its subsequent fertility problems.
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
We present a hypothesis whereby a number of environmental, lifestyle and clinical factors conspire to induce oxidative DNA damage in the male germ line which then triggers the formation de novo mutations which can have a major impact on the health of the offspring including their subsequent fertility.
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
Abstract Spermatogonial stem cells (SSCs) are very sensitive to chemotherapy and radiotherapy, so male infertility is a great challenge for prepubertal cancer survivors. Cryoconservation of testicular cells before cancer treatment can preserve SSCs from treatment side effects. Different two-dimensional (2D) and three-dimensional (3D) culture systems of SSCs have been used in many species as a useful technique to in vitro spermatogenesis. We evaluated the proliferation of SSCs in 2D and 3D culture systems of platelet-rich plasma (PRP). testicular cells of four brain-dead patients cultivated in 2D pre-culture system...
Source: Acta Histochemica - Category: Biochemistry Authors: Tags: Acta Histochem Source Type: research
AbstractStudy questionAre de novo mutations in the human genome associated with male infertility?Summary answerWe identified de novo mutations in five candidate genes:SEMA5A,NEURL4,BRD2, CD1D, andCD63.What is known alreadyEpidemiological and genetic studies have consistently indicated contribution of genetic factors to the etiology of male infertility, suggesting that more than 1500 genes are involved in spermatogenesis.Study design, size, durationFirst, we searched for de novo mutations in patients with idiopathic azoospermia with whole ‐exome sequencing (WES). To evaluate the potential functional impact of de novo iden...
Source: Andrology - Category: Urology & Nephrology Authors: Tags: ORIGINAL ARTICLE Source Type: research
Klinefelter syndrome (KS) is the most common genetic abnormality present in infertile men, and the vast majority of nonmosaic KS patients will have nonobstructive azoospermia (NOA). Microdissection testicular sperm extraction (mTESE) is one of the most effective methods of obtaining testicular sperm, with approximately 50% of men with KS having sperm found at mTESE. Interventions that can optimize sperm retrieval rates (SRR), or at least more accurately guide preoperative counseling, would be of great value to the treatment of men with KS and NOA.
Source: Fertility and Sterility - Category: Reproduction Medicine Authors: Tags: Reflections Source Type: research
ConclusionThis result suggests that formation of the blood-testis barrier is disturbed in affected tubules. To our knowledge this is the first observation of excessive, non-malignant proliferation of spermatogonia in a NOA patient. Although underlying molecular mechanisms remain to be elucidated, we hypothesize that the unusual pathology was triggered by the high-dose clomiphene citrate treatment preceding testicular biopsy.
Source: Basic and Clinical Andrology - Category: Urology & Nephrology Source Type: research
AbstractStudy questionAre de novo mutations in the human genome associated with male infertility?Summary answerWe identified de novo mutations in five candidate genes: SEMA5A, NEURL4, BRD2, CD1D, and CD63.What is known alreadyEpidemiological and genetic studies have consistently indicated contribution of genetic factors to the etiology of male infertility, suggesting that more than 1500 genes are involved in spermatogenesis.Study design, size, durationFirst we searched for de novo mutations in patients with idiopathic azoospermia with whole exome sequencing (WES). To evaluate the potential functional impact of de novo iden...
Source: Andrology - Category: Urology & Nephrology Authors: Tags: ORIGINAL ARTICLE Source Type: research
ConclusionOur results show variants rs2477686 and rs10842262 to be significantly associated with male infertility in the Serbian population. Nevertheless, case-control studies in other populations are needed to validate their association with infertility in males diagnosed with oligoasthenozoospermia and non-obstructive azoospermia.
Source: Journal of Assisted Reproduction and Genetics - Category: Reproduction Medicine Source Type: research
Abstract The role of the male factors in the couple's infertility has been significantly increased in recent years due to a sententious assessment of male reproductive functions and enhanced diagnostic tools. We investigated the correlations among the seminal plasma (SP) levels of each of zinc, testis-expressed sequence 101 (TEX101), and free amino acids levels with reproductive hormones in adult fertile and infertile men. The study included 100 infertile men categorized into 50 non-obstructive azoospermic patients and 50 patients with idiopathic oligoasthenoteratozoospermia (iOAT), in addition to 50 fertile contr...
Source: Biological Trace Element Research - Category: Biology Authors: Tags: Biol Trace Elem Res Source Type: research
More News: Azoospermia | ICSI (Intracytoplasmic Sperm Injection) | Infertility | Reproduction Medicine | Ultrasound