Methods and Process Optimization for Large-Scale CAR T Expansion Using the G-Rex Cell Culture Platform.

Methods and Process Optimization for Large-Scale CAR T Expansion Using the G-Rex Cell Culture Platform. Methods Mol Biol. 2020;2086:165-177 Authors: Ludwig J, Hirschel M Abstract The G-Rex cell culture platform is based on a gas-permeable membrane technology that provides numerous advantages over other systems. Conventional bioreactor platform technologies developed for large scale mammalian cell expansion are typically constrained by the mechanics of delivering oxygen to an expanding cell population. These systems often utilize complex mechanisms to enhance oxygen delivery, such as stirring, rocking, or perfusion, which adds to expense and increases their overall risk of failure. On the other hand, G-Rex gas-permeable membrane-based bioreactors provide a more physiologic environment and avoid the risk and cost associated with more complex systems. The result is a more robust, interacting cell population established through unlimited oxygen and nutrients that are available on demand. By removing the need to actively deliver oxygen, these bioreactors can hold larger medium volumes (more nutrients) which allows the cells to reach a maximum density without complexity or need for media exchange. This platform approach is scaled to meet the needs of research through commercial production with a direct, linear correlation between small and large devices. In the G-Rex platform, examples of cell expansion (9-14 day duration) include; CAR-T cells, which have...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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Source: Frontiers in Neurology - Category: Neurology Source Type: research
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Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Brief Report Source Type: research
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Source: Biomedizinische Technik/Biomedical Engineering - Category: Biomedical Engineering Tags: Biomed Tech (Berl) Source Type: research
Condition:   Cesarean Section Complications Intervention:   Device: Masimo Radical-7® Pulse CO-Oximeter® Sponsor:   Antalya Training and Research Hospital Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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