Methods and Process Optimization for Large-Scale CAR T Expansion Using the G-Rex Cell Culture Platform.

Methods and Process Optimization for Large-Scale CAR T Expansion Using the G-Rex Cell Culture Platform. Methods Mol Biol. 2020;2086:165-177 Authors: Ludwig J, Hirschel M Abstract The G-Rex cell culture platform is based on a gas-permeable membrane technology that provides numerous advantages over other systems. Conventional bioreactor platform technologies developed for large scale mammalian cell expansion are typically constrained by the mechanics of delivering oxygen to an expanding cell population. These systems often utilize complex mechanisms to enhance oxygen delivery, such as stirring, rocking, or perfusion, which adds to expense and increases their overall risk of failure. On the other hand, G-Rex gas-permeable membrane-based bioreactors provide a more physiologic environment and avoid the risk and cost associated with more complex systems. The result is a more robust, interacting cell population established through unlimited oxygen and nutrients that are available on demand. By removing the need to actively deliver oxygen, these bioreactors can hold larger medium volumes (more nutrients) which allows the cells to reach a maximum density without complexity or need for media exchange. This platform approach is scaled to meet the needs of research through commercial production with a direct, linear correlation between small and large devices. In the G-Rex platform, examples of cell expansion (9-14 day duration) include; CAR-T cells, which have...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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ConclusionThe use of IVIM parameters does not improve the characterization of NVNFSTT by comparison with conventional monoexponential ADC calculation.
Source: Diagnostic and Interventional Imaging - Category: Radiology Source Type: research
This study aimed at developing technical recommendations for the acquisition, processing and analysis of renal ASL data in the human kidney at 1.5  T and 3 T field strengths that can promote standardization of renal perfusion measurements and facilitate the comparability of results across scanners and in multi-centre clinical studies.MethodsAn international panel of 23 renal ASL experts followed a modified Delphi process, including on-line surveys and two in-person meetings, to formulate a series of consensus statements regarding patient preparation, hardware, acquisition protocol, analysis steps and data reporti...
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Source: Frontiers in Neurology - Category: Neurology Source Type: research
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Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Brief Report Source Type: research
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Authors: Umapathy S, Thulasi R, Gupta N, Sivanadhan S Abstract The aim and objectives of this study were as follows: (i) to perform automated segmentation of knee thermal image using the regional isotherm-based segmentation (RIBS) algorithm and segmentation of ultrasound image using the image J software; (ii) to implement the RIBS algorithm using computer-aided diagnostic (CAD) tools for classification of rheumatoid arthritis (RA) patients and normal subjects based on feature extraction values; and (iii) to correlate the extracted thermal imaging features and colour Doppler ultrasound (CDUS) features in the knee re...
Source: Biomedizinische Technik/Biomedical Engineering - Category: Biomedical Engineering Tags: Biomed Tech (Berl) Source Type: research
Condition:   Cesarean Section Complications Intervention:   Device: Masimo Radical-7® Pulse CO-Oximeter® Sponsor:   Antalya Training and Research Hospital Recruiting
Source: - Category: Research Source Type: clinical trials
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Source: Journal of Neurosurgical Anesthesiology - Category: Anesthesiology Tags: Review Articles Source Type: research
AbstractPurpose of reviewEndovascular thrombectomy (ET), the standard of treatment for emergent large vessel occlusion (ELVO) strokes, has been subject to rigorous efforts to further improve its usage and delivery for optimised patient outcomes. This review aims to provide an outline and discussion about the recently established and emerging recommendations regarding endovascular treatment of stroke.Recent findingsThe indications for ET have expanded continually, with perfusion imaging now enabling selection of patients presenting 6 –24 h after last-known-well, and improved device and operator proficiency allowi...
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