Accelerated Intestinal Epithelial Cell Turnover Correlates with Stimulated BMP Signaling Cascade following Intestinal Ischemia –Reperfusion in a Rat

Eur J Pediatr Surg DOI: 10.1055/s-0039-1700550 Introduction Bone morphogenetic proteins (BMPs) are a family of proteins that regulate proliferation and differentiation of intestinal epithelial cells. The purpose of this study was to evaluate the role of BMP signaling following intestinal ischemia–reperfusion (IR) in a rat model. Materials and Methods Male Sprague-Dawley rats were divided into four experimental groups: Sham-24 and Sham-48 rats underwent laparotomy and were sacrificed 24 or 48 hours later, respectively; IR-24 and IR-48 rats underwent occlusion of superior mesenteric artery and portal vein for 30 minutes followed by 24 or 48 hours of reperfusion, respectively. Enterocyte proliferation and apoptosis were determined at sacrifice. BMP-related genes and protein expression were determined using real-time polymerase chain reaction, Western blot, and immunohistochemistry for 48 hours followed by IR. Results IR rats demonstrated a significant increase in BMP2 (twofold increase, p 
Source: European Journal of Pediatric Surgery - Category: Surgery Authors: Tags: Original Article Source Type: research

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ConclusionPreoperative, immediate postoperative and 24-h postoperative lactate value independently predict 28-day mortality in perforation peritonitis patients undergoing emergency laparotomy. Combination of MPI and 24-h lactate value is the most accurate predictor of mortality.Trial registrationClinical Trial Registry of India -CTRI/2018/01/011103
Source: Journal of Intensive Care - Category: Intensive Care Source Type: research
In this study, we compared different anesthesia and operation methods for modeling VX2 hepatocarcinoma in rabbits. Forty New Zealand white rabbits were randomly divided into three groups: A, B, and C. Group A underwent ultrasound-guided implantation and intravenous anesthesia; Group B underwent ultrasound-guided implantation and inhalation anesthesia; Group C underwent laparotomy implantation and intravenous anesthesia. Anesthesia and operation differences were compared between groups A and B, and A and C, respectively. We used magnetic resonance imaging (MRI) to assess tumor formation and growth, and pathological examinat...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
We present a case of a 58-year-old female with anti-PD-1 immunotherapy-related small bowel perforation. The patient was on long-term therapy with nivolumab for metastatic non-small cell lung cancer. She presented to the emergency department with acute abdominal pain, in which the CT revealed a short segment of dilated distal ileum proximal to a very short segment of bowel with mural thickening and a perforation near the transition point. The patient underwent subsequent laparotomy, which confirmed the CT findings and revealed a short-segment of friable and dilated loop of distal ileum proximal to a stricture and a small pe...
Source: Emergency Radiology - Category: Emergency Medicine Source Type: research
An Analysis of the Decision-Making Process After "Decision not to Operate" in Acutely Unwell, High-Risk General Surgery Patients. Am J Hosp Palliat Care. 2019 Dec 09;:1049909119893598 Authors: Pinto-Lopes R, Thahir A, Halahakoon VC Abstract OBJECTIVES: The purpose of this study was to analyze the decision-making process in emergency general surgery in an attempt to ascertain whether surgeons make the correct decision when decisions not to operate in high-risk acutely unwell surgical patients are taken. BACKGROUND: A decision not to operate is sometimes associated with a certain degree o...
Source: The American Journal of Hospice and Palliative Care - Category: Palliative Care Authors: Tags: Am J Hosp Palliat Care Source Type: research
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Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
Conclusion: The results suggest that pre- and post-treatment with EGCG may be effective in protecting spinal cord from IRI. PMID: 31809244 [PubMed - as supplied by publisher]
Source: Journal of Spinal Cord Medicine - Category: Orthopaedics Tags: J Spinal Cord Med Source Type: research
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Source: The American Journal of Surgery - Category: Surgery Source Type: research
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Source: Obstetrics and Gynecology - Category: OBGYN Authors: Tags: Obstet Gynecol Source Type: research
Several multicenter randomized controlled trials comparing laparoscopy and conventional open surgery for colon cancer have demonstrated that laparoscopic approach achieved the same oncological results while im...
Source: BMC Cancer - Category: Cancer & Oncology Authors: Tags: Study protocol Source Type: research
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Source: Journal of Cardiac Surgery - Category: Cardiovascular & Thoracic Surgery Authors: Tags: CASE REPORT Source Type: research
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