Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656

In this study, we focused on mating-type switching and gene expression in order to elucidate its sexual life cycle and establish genetic approaches applicable for the strain. A putative mating-type gene cluster was identified in TBRC656 that is syntenic to the cluster in Ogataea parapolymorpha DL-1 (previously named Hansenula polymorpha). Like DL-1, TBRC656 possesses two mating loci, namely MATa and MATα, and also shows flip-flop mating-type switching. Interestingly, unlike any other methylotrophic yeast, TBRC656 robustly switched mating type during late growth in rich medium (YPD). Under nutrient depletion, mating-type switching was observed within one hour. Transcription from both MATa and MATα mating loci was detected during growth in YPD, and possibly induced upon nitrogen depletion. Gene expression from MATα was detected as a single co-transcript from a three-gene array (α2-α1-a1S). Deletion of a putative a1S ORF at the MATα locus had no observed effect on mating-type switching but demonstrated significant effect on mating-type gene expression at both MATa and MATα loci.
Source: Microbiological Research - Category: Infectious Diseases Source Type: research

Related Links:

Publication date: June 2020Source: Journal of Bone Oncology, Volume 22Author(s): Michala Skovlund Sørensen
Source: Journal of Bone Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 4 April 2020Source: Journal of Geriatric OncologyAuthor(s): Kelly M. Trevino, Amy Stern, Holly G. Prigerson
Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 4 April 2020Source: Journal of Geriatric OncologyAuthor(s): Isacco Desideri, Carlotta Becherini, Luca Visani, Giulia Stocchi, Erika Scoccimarro, Matteo Mariotti, Viola Salvestrini, Silvia Scoccianti, Daniela Greto, Pierluigi Bonomo, Guido Pecchioli, Lorenzo Bordi, Alessandro Della Puppa, Giulio Francolini, Icro Meattini, Lorenzo Livi
Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 4 April 2020Source: Journal of Geriatric OncologyAuthor(s): Joshua B. Cadwell, Anoushka M. Afonso, Armin Shahrokni
Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 4 April 2020Source: Journal of Geriatric OncologyAuthor(s): Claire A. Surr, Rachael Kelley, Alys W. Griffiths, Laura Ashley, Fiona Cowdell, Ann Henry, Michelle Collinson, Ellen Mason, Amanda J. Farrin
Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 3 April 2020Source: Anaesthesia Critical Care &Pain MedicineAuthor(s): Brajesh Kaushal, Sandeep Chauhan, Rohan Magoon, Nitish Naik, Ambuj Roy
Source: Anaesthesia, Critical Care and Pain Medicine - Category: Anesthesiology Source Type: research
Publication date: Available online 3 April 2020Source: Anaesthesia Critical Care &Pain MedicineAuthor(s): Frederic J. Baud, Vincent Jullien, Philippe-Henri Secrétan, Pascal Houzé, Lionel Lamhaut
Source: Anaesthesia, Critical Care and Pain Medicine - Category: Anesthesiology Source Type: research
Publication date: Available online 3 April 2020Source: Anaesthesia &Intensive Care MedicineAuthor(s): Viyayanand Nadella
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
Publication date: Available online 12 March 2020Source: The Journal of Allergy and Clinical Immunology: In PracticeAuthor(s): Karsten Weller, Tamara Donoso, Markus Magerl, Emel Aygören-Pürsün, Petra Staubach, Inmaculada Martinez-Saguer, Tomasz Hawro, Sabine Altrichter, Karoline Krause, Frank Siebenhaar, Martin Metz, Torsten Zuberbier, Denise Freier, Marcus Maurer
Source: The Journal of Allergy and Clinical Immunology: In Practice - Category: Allergy & Immunology Source Type: research
Publication date: Available online 3 April 2020Source: Autoimmunity ReviewsAuthor(s): Dennis McGonagle, Kassem Sharif, Anthony O'Regan, Charlie Bridgewood
Source: Autoimmunity Reviews - Category: Allergy & Immunology Source Type: research
More News: Genetics | Infectious Diseases | Microbiology | Nutrition | Study