De novo Biosynthesis of Indole-3-ethanol and Indole-3-ethanol acetate in Engineered Escherichia coli

In this study, we established an artificial pathway in engineered Escherichia coli for microbial production of indole-3-ethanol from simple carbon sources. The overall pathway includes the upstream pathway from glucose to L-tryptophan and the downstream pathway from L-tryptophan to indole-3-ethanol. We have further demonstrated the biosynthesis of indole-3-ethanol acetate by overexpressing alcohol acetyltransferase ATF1 for the subsequent conversion of indole-3-ethanol to indole-3-ethanol acetate. To our knowledge, this is the first report on the biosynthesis of indole-3-ethanol and indole-3-ethanol acetate directly from a renewable carbon source.Graphical abstract
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research