High glucose inhibits osteogenic differentiation and proliferation of MC3T3 ‑E1 cells by regulating P2X7.

High glucose inhibits osteogenic differentiation and proliferation of MC3T3‑E1 cells by regulating P2X7. Mol Med Rep. 2019 Oct 31;: Authors: Yang J, Ma C, Zhang M Abstract Diabetes mellitus adversely affects human bones and increases the risk of developing osteoporosis. In the present study, treatment with 30 mmol/l glucose was used to establish a high glucose (HG) cell model in vitro. Plasmids were used to overexpress the P2X purinoceptor 7 (P2X7) gene. Brilliant blue G and (4‑benzoyl‑benzoyl)‑ATP were used as a P2X7 antagonist and agonist, respectively. Proliferation of osteogenic MC3T3‑E1 cells and alkaline phosphatase (ALP) activity were determined using MTT and colorimetric assays, respectively. Alizarin Red S was used to assess calcification of MC3T3‑E1 cells. Western blotting and reverse transcription‑quantitative PCR were performed to determine protein and mRNA expression levels. The results demonstrated that HG inhibited MC3T3‑E1 cell proliferation and P2X7 expression, reduced calcification, and downregulated the expression levels of ALP and osteocalcin (Ocn) in MC3T3‑E1 cells. Overexpression of P2X7 in HG conditions increased calcification and proliferation, and upregulated the levels of ALP and Ocn in MC3T3‑E1 cells. Inhibition of P2X7 downregulated the expressions of ALP and Ocn in MC3T3‑E1 cells under HG conditions. Therefore, the present results indicated that HG caused damage to osteogenic MC3T3‑E1 cells. T...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research

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AbstractIntroductionLow body weight and hypoalbuminemia were independently associated with osteoporosis, the relationship among Geriatric Nutritional Risk Index (GNRI), Bone Mineral Density (BMD) and osteoporosis in Type 2 Diabetes Mellitus (T2DM) was explored and the GNRI predictive value was evaluated.Materials and MethodsWe enrolled 225 men and 192 women suffered from T2DM. Their general condition, laboratory, and BMD data were collected. Spearman ’s partial correlation analysis adjusting for age, BMI and albumin was used for exploring the association among GNRI, BMD and bone metabolism markers. Statistical analys...
Source: Journal of Diabetes Investigation - Category: Endocrinology Authors: Tags: ORIGINAL ARTICLE Source Type: research
We present the case of a 59-year-old woman with third-grade obesity and severe comorbidities including osteoporosis, dyslipidemia, diabetes mellitus, hypertension, night eating following bariatric biliary-intestinal bypass surgery, severe fibromyalgia, poly-arthrosis, lumbar disc herniation in L5S1, sleep disorders and sleep apnea syndrome, and emotional disorders with anxiety and depression, who suffered from chronic pain unresponsive to a combination of multiple analgesics. After a period of metabolic and nutritional rehabilitation, analgesic treatment with tapentadol prolonged release (PR) was started and gradually incr...
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
AbstractPurpose of ReviewIndividuals with type 1 and type 2 diabetes mellitus (T1DM, T2DM) have an increased risk of bone fracture compared to non-diabetic controls that is not explained by differences in BMD, BMI, or falls. Thus, bone tissue fracture resistance may be reduced in individuals with DM. The purpose of this review is to summarize work that analyzes the effects of T1DM and T2DM on bone tissue compositional and mechanical properties.Recent FindingsStudies of clinical T2DM specimens revealed increased mineralization and advanced glycation endproduct (AGE) concentrations and significant relationships between mecha...
Source: Current Osteoporosis Reports - Category: Orthopaedics Source Type: research
Authors: Giovos G, Yavropoulou MP, Yovos JG Abstract The improving effectiveness of health care leads inevitably to a rapid increase in the elderly population worldwide. At advanced ages, however, people experience chronic disabilities, which significantly increase the social and economic burden while curtailing survival, independence, and quality of life of the aging population. As aging is a multifactorial process, apart from genetic predisposition, other environmental factors, such as chronic sterile inflammation and cellular senescence, contribute as crucial participants and have been targeted to reverse their ...
Source: Hormones - Category: Endocrinology Tags: Hormones (Athens) Source Type: research
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Source: Sao Paulo Medical Journal - Category: General Medicine Authors: Tags: Sao Paulo Med J Source Type: research
Abstract The skeleton harbors an array of lineage cells that have an essential role in whole body homeostasis. Adipocytes start the colonization of marrow space early in postnatal life, expanding progressively and influencing other components of the bone marrow through paracrine signaling. In this unique, closed, and hypoxic environment close to the endosteal surface and adjacent to the microvascular space the marrow adipocyte can store or provide energy, secrete adipokines, and target neighboring bone cells. Adipocyte progenitors can also migrate from the bone marrow to populate white adipose tissue, a process th...
Source: Annual Review of Physiology - Category: Physiology Authors: Tags: Annu Rev Physiol Source Type: research
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Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
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Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
Authors: Ding X, Yang L, Hu Y, Yu J, Tang Y, Luo D, Zheng L Abstract Type 2 diabetes mellitus (T2DM), a leading cause of osteoporosis, remains a contraindication for bone implant therapy. Although associated with side effects when systemically administered, biphosphonates (BPs) play a positive role in diabetic osteoporosis treatment. We hypothesized that local BP therapy would prevent decayed implant osseointegration under T2DM conditions. To assess cell proliferation and determine the optimal BP concentration, bone marrow-derived mesenchymal stem cells (BMSCs) and bone marrow macrophages (BMMs) were treated with B...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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Source: Osteoporosis International - Category: Orthopaedics Source Type: research
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