Antimicrobial, anti-adherence and antibiofilm activity against Staphylococcus aureus of a 4-phenyl coumarin derivative isolated from Brazilian geopropolis

Publication date: Available online 6 November 2019Source: Microbial PathogenesisAuthor(s): Marcos Guilherme da Cunha, Janaina de Cássia Orlandi Sardi, Irlan Almeida Freires, Marcelo Franchin, Pedro Luiz RosalenAbstractThe discovery of new drug candidates, especially from natural products, remains a promising approach to overcome the alarmingly high microbial resistance rates. A major 4-phenyl coumarin named cinnamoyloxy-mammeisin (CNM) isolated from stingless bee geopropolis showed interesting biological properties; however, its antimicrobial activity against Staphylococcus aureus has never been investigated. In order to clarify these properties, CNM isolated from geopropolis was initially tested against methicillin-susceptible and -resistant S. aureus strains. Further, the effects of CNM were assessed on the microbial adherence to human cells, biofilm formation and mature biofilm. Then, the acute toxicity of the compound was determined in Galleria mellonella. CNM showed bacteriostatic activity against methicillin-susceptible and -resistant S. aureus strains, with MIC of 11.3 μM. In addition, CNM at 5.7 μM reduced bacterial adherence to human keratinocytes from 1 to 3h and disrupted biofilm formation by reducing cell viability and architecture, as evidenced by scanning electron microscopy. The acute toxicity assay indicated no significant harmful effects. Based on these findings, CNM can be considered a promising compound with anti-S. aureus properties and pr...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

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Publication date: Available online 3 December 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Kandasamy Saravanakumar, Elango Jeevithan, Xiaowen Hu, Ramachandran Chelliah, Deog-Hwan Oh, Myeong-Hyeon WangAbstractThis work reports the optimization, synthesis, characterization, anticancer, and antibacterial activity of the Trichoderma-β-D-glucan‑zinc oxide nanoparticles (T-β-D-glu-ZnO NPs). Firstly, the T-ZnO NPs was synthesized using the fungal mycellial water extract (FWME) derived from T. harzianum strain SKCGW009 under the optimized condition of extract concentration (5.99 mL), te...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Tin oxide (SnO2) nanoparticles were synthesised using various surfactants of different charges (n-cetyl trimethyl ammonium bromide, sodium dodecyl sulphate and TRITON X-100) by the co-precipitation method. The synthesised nanomaterials were characterised using different techniques to study their structural, surface morphological, optical and anti-bacterial activities. X-ray diffraction patterns revealed the formation of a tetragonal rutile structure in pure and surfactants-aided SnO2 nanoparticles and the results show good agreement with JCPDS data [41-1445]. The crystallite size of SnO2 nanoparticles was found to decrease...
Source: IET Nanobiotechnology - Category: Nanotechnology Source Type: research
Authors: Hartinger JM, Lukáč P, Mitáš P, Mlček M, Popková M, Suchý T, Šupová M, Závora J, Adámková V, Benáková H, Slanař O, Šíma M, Bartoš M, Chlup H, Grus T Abstract The study presents a novel vancomycin-releasing collagen wound dressing derived from Cyprinus carpio collagen type I cross-linked with carbodiimide which retarded the degradation rate and increased the stability of the sponge. Following lyophilization, the dressings were subjected to gamma sterilization. The structure was evaluated via scanning ...
Source: Bosnian Journal of Basic Medical Sciences - Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research
In this study, we sequenced and characterized the goose-type lysozyme gene, termed as BsLysG, from the Chinese black sleeper (Bostrychus sinensis). The BsLysG encodes 196 amino acids and contains a soluble bacterial lytic transglycosylases domain, three catalytic residues (Glu72, Asp85 and Asp102) and the GLMQ motif (Gly97, Leu98, Met99 and Gln100). No signal peptide was observed in the BsLysG protein. The genomic DNA of BsLysG contains five exons and four introns. The sequence analyses showed that the BsLysG exhibits high similarity with LysG from other fishes. Phylogenetic analyses showed that the BsLysG is clustered tog...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: Available online 29 November 2019Source: Microbial PathogenesisAuthor(s): Shouping Zhang, Bin Hu, Jingfei Xu, Qiuxuan Ren, Zhichen Wang, Sanhu Wang, Yongjun Dong, Guoyu YangAbstractA Staphylococcus aureus (S.aureus) was isolated from pigs suffered in pneumonia that can't be cured by antibiotic such as methicillin and vancomycin. It was demonstrated that baicalin, an active natural compound extracted from the traditional Chinese medicinal, possess antimicrobial activity. In the present study, we evaluate it efficacy in vitro and vivo against this isolated methicillin-resistant S.aureus (MRSA). Our findings...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: March 2020Source: Materials Science and Engineering: C, Volume 108Author(s): Yunan Qing, Kaishen Li, Dongdong Li, Yanguo QinAbstractFunctionalization of porous metals with antibacterial coatings is hotly pursued in recent decade. Here we fabricated a highly porous stainless steel component by selective laser melting and then coated with silver incorporated zeolite by in situ hydrothermal crystallization method. The morphology of their surface was investigated by scanning electron microscopy. The inhibition of Escherichia coli and Staphylococcus aureus were identified by bacterial viability studies after 2...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 28 November 2019Source: European Polymer JournalAuthor(s): Sumita Swar, Veronika Máková, Jana Horáková, Pavel Kejzlar, Petr Parma, Ivan StiborAbstractHospital-acquired infections (HAI) caused by pathogenic bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus adversely affect public health due to their colonization and subsequent formation of a biofilm on medical devices. Various functionalized polymers including polyamides are commonly used in the biomedical industry aiming to resist bacterial infection as well as to ensure cytocompatibility of the...
Source: European Polymer Journal - Category: Chemistry Source Type: research
Anti-biofilm Effect of β-Lapachone and Lapachol Oxime Against Isolates of Staphylococcus aureus. Curr Microbiol. 2019 Nov 29;: Authors: Fernandes AWC, Dos Anjos Santos VL, Araújo CRM, de Oliveira HP, da Costa MM Abstract Antimicrobial resistance in bacteria, such as Staphylococcus aureus, has been the subject of many assistance studies of alternatives for the treatment of infections. These studies aim to solve this problem for bacteria, such as biofilm formation. Aiming to control the emergence of the problem or enhance antibiotic activity, the data sources are inserted into new therapeuti...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
Publication date: Available online 27 November 2019Source: Polymer TestingAuthor(s): Yasir Anwar, Khalid M. AlghamdiAbstractBiosynthesis of metallic nanoparticles has been always remained important because the process does not involve any synthetic harmful agent. In this research work, silver (Ag) nanoparticles were prepared using fresh peel juice of the Citrus sinensis fruit. The obtained nanoparticles were characterized using spectroscopic techniques of field emission scanning electron microscopy, X-ray diffraction, and uv–visible spectroscopy. After characterization, the synthesized Ag nanoparticles were used in a...
Source: Polymer Testing - Category: Chemistry Source Type: research
In this study a novel and smart multi-functional hydrogel (MFH) was synthesized from N, N dimethyl acrylamide (DMAAm), gelatin, citric acid (CA) and pomegranate extract (PE) for instant and easy monitoring of the color change in MFH due to changes in medium conditions such as pH and temperature. MFH was utilized as food packaging material, equipped with the developed properties. MFH was synthesized with a redox polymerization technique in film form on petri dishes. Mechanical and water resistance properties of MFH were improved by CA and N, N, methylenebisacrylamide, while PE was used to gain antimicrobial, antioxidant and...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
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