Identification of genes related to resistance against S. typhimurium in ovine macrophages

In this study, sixty significant differentially expressed genes were screened between susceptible and resistant sheep macrophages by transcriptome RNA-seq. Eight significantly enriched GO terms and six canonical pathways were involved by GO and KEGG enrichment analysis. Furthermore, the function of HMOX1, SLPI and SPP1 was verified by siRNA, which the knockdown of HMOX1 and SLPI increased remarkably the clearance of S. typhimurium, but SPP1 had little effect on the clearance of S. Typhimurium within sheep macrophages. Altogether, these results suggest that many genes of macrophages were reprogrammed via S. Typhimurium infected, some of which may facilitate host defence against Salmonella, while others allow Salmonella to escape.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

Related Links:

The objective of this study was to determine whether deletion of the sseK3 gene can attenuate the virulence of S. Typhimurium. To do this, we constructed an sseK3 deletion mutant using the double-exchange allele of the suicide plasmid pRE112ΔsseK3 and assessed the virulence and intracellular proliferation of the mutant. The sseK3 deletion mutant exhibited adhesion and invasion properties similar to those of wild-type (WT) S. Typhimurium, although the virulence and intracellular proliferation of the mutant were significantly reduced compared to that of the WT strain. Furthermore, the observed increase in the median le...
Source: Canadian Journal of Veterinary Research - Category: Veterinary Research Authors: Tags: Can J Vet Res Source Type: research
This study is the first to demonstrate that bacteria grown in the presence of catecholamine stress hormones alter their growth environment, probably by producing immunomodulating substances, in a way that host immune response is suppressed. These findings add a new dimension to interkingdom signaling and provide novel clues to explain the increased susceptibility of a stressed host to Salmonella infection.
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Abstract Streptococcus suis, a major zoonotic pathogen in swine, can be classified into 35 serotypes. However, no universal vaccine against the multiple serotypes of S. suis is available, though some studies have shown homologous protection. Hence, developing an effective universal vaccine to protect pigs against multiple S. suis serotypes is necessary, or at the very least, to protect pigs against diseases caused by the dominant pathogenic serotypes. Enolase, a highly conserved surface protein, is present in all of the described S. suis serotypes. rSC0016 is an improved recombinant attenuated S. Choleraesuis vacc...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
Abstract Background: The 21st century has seen a wide range of diseases resulting from zoonotic infections, of which bacterial infections have led to outbreaks of food-borne diseases. Aim: The study looks at bacterial pathogen carriage by farm rats and their antimicrobial susceptibility, with the view of providing insights for antimicrobial surveillance. Method: Farm rats of Rattus rattus species where randomly collected alive from farms in Al-Ahsa using food baits. They were anaesthetize with urethane within 4 h of collection and were unconscious for the collection of samples. Basic bacteriologic...
Source: Environmental Health Insights - Category: Environmental Health Authors: Tags: Environ Health Insights Source Type: research
In conclusion, this is the first report of co-infection of SE with H9N2-AIV in chickens, which leads to increased pathogenicity, SE faecal shedding and organ colonization, and H9N2-AIV shedding titre and duration, resulting in substantial economic losses and environmental contamination, ultimately leading to increased zoonoses. PMID: 32835500 [PubMed - in process]
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
Salmonella Weltevreden is increasingly reported from aquatic environments, seafood, and patients in several Southeast Asian countries. Using genome-wide analysis, we characterized S. Weltevreden isolated from cultured shrimp and tilapia from Vietnam and China to study their genetic characteristics and relatedness to clinical isolates of S. Weltevreden ST-365. The phylogenetic analysis revealed up to 312 single-nucleotide polymorphism (SNP) difference between tilapia isolates, whereas isolates from shrimp were genetically more closely related. Epidemiologically unrelated isolates from Vietnam were closely related to isolate...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Salmonella is an important zoonotic pathogen, and chickens are one of its main hosts. Every year, Salmonella infections pose a serious threat to the poultry industry in developing countries, especially China. In ...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
Although salmonellosis is considered one of the most important food-borne zoonotic diseases in Europe, close contact between dogs and their owners can also be a potential source of Salmonella spp. for humans. Thi...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
Salmonellosis is an important food borne disease of public health significance. Global estimates of the disease burden shows more than 20 million cases and 0.15 million deaths annually. The disease caused by a variety ofSalmonella organisms worldwide.Salmonella pathogens are belonging to family Enterobacteriaceae that are known to infect many hosts inducing variable clinical diseases pictures. Typhoidal and non-typhoidalSalmonellae are common diseases among Egyptians with severe socioeconomic losses. Different species of animals and poultry as well as their products are the main sources and reservoirs for zoonotic human il...
Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
Abstract Salmonella enterica serovar Hvittingfoss is an important foodborne serotype of Salmonella, being detected in many countries where surveillance is conducted. Outbreaks can occur, including a recent multi-state foodborne outbreak in Australia. S Hvittingfoss can be found in animal populations, though a definitive animal host has not been established. Six species of birds were sampled at Roebuck Bay, a designated Ramsar site in north-western Australia, resulting in 326 cloacal swabs for bacterial culture. A single flock of 63 Bar-tailed Godwit (Limosa lapponica menzbieri) caught at Wader Spit, Roebuck Bay in...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
More News: Genetics | Infectious Diseases | International Medicine & Public Health | Men | Salmonella | Study | Zoonoses