Identification of genes related to resistance against S. typhimurium in ovine macrophages

In this study, sixty significant differentially expressed genes were screened between susceptible and resistant sheep macrophages by transcriptome RNA-seq. Eight significantly enriched GO terms and six canonical pathways were involved by GO and KEGG enrichment analysis. Furthermore, the function of HMOX1, SLPI and SPP1 was verified by siRNA, which the knockdown of HMOX1 and SLPI increased remarkably the clearance of S. typhimurium, but SPP1 had little effect on the clearance of S. Typhimurium within sheep macrophages. Altogether, these results suggest that many genes of macrophages were reprogrammed via S. Typhimurium infected, some of which may facilitate host defence against Salmonella, while others allow Salmonella to escape.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research