Enhancement of host infectivity, immunity, and protective efficacy by addition of sodium bicarbonate antacid to oral vaccine formulation of live attenuated Salmonella secreting Brucella antigens

Publication date: January 2020Source: Microbial Pathogenesis, Volume 138Author(s): Chamith Hewawaduge, Amal Senevirathne, John Hwa LeeAbstractIn the present study, the importance of sodium bicarbonate antacid as an agent for an orally delivered attenuated Salmonella strain secreting Brucella antigens Cu–Zn superoxide dismutase (SodC) and outer membrane protein 19 (Omp19) as a live vaccine candidate against Brucella infection was investigated. First, Brucella antigens SodC and Omp19 were cloned into a prokaryotic constitutive expression vector, pJHL65. Then secretion of proteins was verified after transformation into an attenuated Salmonella typhimurium (ST) strain, JOL1800 (Δlon, ΔcpxR, Δasd, ΔrfaL), using western blot analysis. Mice were orally inoculated with phosphate-buffered saline (PBS) or with a co-mixture Salmonella secreting each antigens at a 1:1 ratio, each containing 1 × 108 CFU/mouse with and without sodium bicarbonate treatment. For antacid treatment, 1.3% w/v sodium bicarbonate was orally administered 30 min before and immediately after immunization with the Salmonella formulation. Humoral and cell-mediated immune responses were evaluated to investigate the efficacy of sodium bicarbonate in an oral formulation. The results indicated that addition of sodium bicarbonate to the vaccine significantly increased (P 
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

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This study aimed to assess the protective efficacy of a novel Brucella vaccine formulation in goats. Twenty black goats were separated into 2 groups. Group A was injected with 3.0 × 109 CFU (colony-forming units)/mL of a Salmonella-based delivery system harboring only vector (pMMP65). Group B was immunized with 3.0 × 109 CFU/mL of the vaccine, a mixture of 3 Brucella vaccine strains (using a Salmonella-based delivery system) expressing each recombinant B. abortus Omp3b, BCSP31, and SOD protein. No Salmonella delivery strain was isolated from all tested lymph nodes and parenchymal organs. Serum immunoglobulin (I...
Source: Canadian Journal of Veterinary Research - Category: Veterinary Research Authors: Tags: Can J Vet Res Source Type: research
In conclusion, we unraveled the secretome of G. adiacens, an oral bacterium well-documented in infective endocarditis, but also recently shown to be involved in oral infections. Importantly, the secretome of G. adiacens comprised of a large number of putative virulence factors. Of particular importance is the finding that the G. adiacens secretome comprised of a number of “moonlighting” proteins, which in other species are shown to enhance bacterial colonization and virulence through their multifunctional roles (Pavkova et al., 2017; Graf et al., 2019). Thus, our results provide a basis for investigating the ro...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Publication date: Available online 14 February 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Amal Senevirathne, Chamith Hewawaduge, John Hwa LeeAbstractWe demonstrated the use of attenuated Salmonella strains secreting Brucella antigens SodC, Omp19, BLS, and PrpA as live vaccine candidates against Brucella abortus infection and presented their cross-protection against Salmonella infections using a BALB/c mice model. Here, a single immunization with each individual strain was capable of establishing significantly high (p 
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research
Abstract Residual virulence is a major drawback in current Brucella vaccines. Live vaccines induce abortions in pregnant animals. Hence, a novel anti-Brucella vaccine was developed utilizing rough Salmonella delivering four Brucella antigens. Safety implications during pregnancy, humoral immune responses, and protective efficacy against wild type Brucella was investigated in guinea pig model. The vaccine did not induce abortions or severe complications in pregnant guinea pigs when administered 4 × 108 CFU via intraperitoneal route. Systemic IgG determination against antigen components reveals induction...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
This study investigated the safety of the vaccine formulation in mice model and in vitro human cell cultures. The experiment was designed to determine the LD50 of the vaccine formulation. The vaccine formulation did not induce any mortality even when mice were administered at 8 × 109 CFU per oral or per subcutaneous (SC), which was 100-times more than the actual vaccine dose intended for mice model. In contrast, wild-type (WT) Salmonella positive control strain induced 100% mortality at 8 × 107 CFU per mice via oral or SC routes. Interaction of the vaccine with phagocytic (THP-1 derived macrophage) and ...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
Publication date: January 2018 Source:Veterinary Immunology and Immunopathology, Volume 195 Author(s): Jonathan Lalsiamthara, John Hwa Lee With an objective to generate safe and effective anti-Brucella vaccine, an attenuated live Salmonella Typhimurium vector delivering heterologous Brucella immunogenic proteins SOD, Omp19, BLS, and PrpA formulated with purified Brucella abortus lipopolysaccharide was evaluated on a guinea pig model. This model represents high susceptibility to Brucella infections and showed similarities in reproducing human pathologies. On safety perspectives, the vaccine formulation induced no observabl...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research
In this study we developed and formulated a combination of four attenuated Salmonella Typhimurium live vector strains secreting heterologous Brucella antigens (rBs), namely lumazine synthase, proline racemase subunit A, lipoprotein outer membrane protein-19, and Cu-Zn superoxide dismutase. With an aim to develop a cross-protecting vaccine, Brucella pan-species conserved rBs were selected. The present study compared the efficacy of smooth and rough variants of Salmonella delivery vector and also evaluated the inclusion of purified Brucella lipopolysaccharide (LPS) in the formulation. Immunization of SPF-BALB/c mice with the...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
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Source: Protein Science - Category: Biochemistry Authors: Tags: Full ‐Length Papers Source Type: research
Gabriela S. Risso, Marianela V. Carabajal, Laura A. Bruno, Andr és E. Ibañez, Lorena M. Coria, Karina A. Pasquevich, Seung-Joo Lee, Stephen J. McSorley, Gabriel Briones, Juliana Cassataro
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 17 July 2015 Source:Veterinary Microbiology Author(s): D. Lacasta, L.M. Ferrer, J.J. Ramos, J.M. González, A. Ortín, G.C. Fthenakis Development and implementation of health management plans is the cornerstone of profitable farms; prevention of microbial diseases by means of vaccination is an integral part of such a plan. In every production type and management system in small ruminants, microbial diseases have a major significance, hence their proper control must be based in good health management practices, including use of effective and safe vaccines. Development o...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
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