Human bioavailability of phenolic compounds found in common beans: the use of high resolution mass spectrometry to evaluate inter individual variability.

Human bioavailability of phenolic compounds found in common beans: the use of high resolution mass spectrometry to evaluate inter individual variability. Br J Nutr. 2019 Nov 08;:1-42 Authors: Mecha E, Feliciano RP, Rodriguez-Mateos A, Silva SD, Figueira ME, Vaz Patto MC, Bronze MR Abstract Although common beans (Phaseolus vulgaris L.) are consumed worldwide, studies on the metabolic fate of phenolic compounds from common beans are still very scarce. The present work aimed to study the bioavailability of phenolic compounds in human plasma and urine, after acute consumption of a single meal of cooked common beans. Blood and urine of seven volunteers were collected before (0 h) and at different time points (1, 2, 4, 6 and 8 h, for plasma and 0-2, 2-4, 4-6, 6-8, 8-24 h for urine) after beans' intake. UPLC-Q-TOF-MS was used for quantification. After beans' intake (405 ± 3 g), containing 457 mg of phenolic compounds (expressed as gallic acid equivalents), there was a significant increase (p < 0.05) in the plasma concentration of 6 metabolites, and in the urinary excretion of 11 metabolites. After 1 h post-consumption, metabolites, such as kaempferol-3-O-glucuronide, showed a significant increase in plasma concentration, suggesting kaempferol's glucuronidation in the upper gastrointestinal tract. More than 50% of the total amount of metabolites, such as 4-methylcatechol-O-sulfate and dihydrocaffeic acid-3-O-sulfate, were excreted after ...
Source: The British Journal of Nutrition - Category: Nutrition Authors: Tags: Br J Nutr Source Type: research