Control of Salmonella in chicken meat using a combination of a commercial bacteriophage and plant-based essential oils

The objectives of this study were to evaluate the antimicrobial activity of 10 essential oil compounds and to determine the combined effects with a commercial bacteriophage (SALMONELEX) against Salmonella. Thymol and carvacrol were the most active compounds tested, with a minimum inhibitory concentration (MIC) of 0.25-1 mg/ml and a minimum bactericidal concentration (MBC) of 0.5-1 mg/ml against three Salmonella strains. Multiplicity of infection (MOI), or bacteriophage/pathogen ratio (PFU/CFU), significantly affected bacteriophage efficacy in media and in chicken products. The bacteriophage decreased Salmonella Typhimurium JWC-3001 by 5 log CFU/ml at low MOI (≥1.7) in tryptic soy broth, but a higher bacteriophage concentration was needed to exhibit bactericidal effect in chicken products. Dipping of inoculated chicken in 1.6 % (w/v) thymol and carvacrol emulsions for 3 min inactivated 1.3 and 1.6 log CFU/g, respectively, of a cocktail of three Salmonella strains. A sequential dipping treatment of inoculated chicken in bacteriophage (1.1×108 PFU/ml) for 3 min and in 1.6% (w/v) thymol or carvacrol for 3 min resulted in reductions of 1.9-2.0 log CFU/g of the cocktail. The combined treatments resulted in significantly greater reduction of Salmonella than individual bacteriophage or essential oil treatments.
Source: Food Control - Category: Food Science Source Type: research

Related Links:

Authors: Parra-Payano VD, Rondón-Paz CR, García C Abstract The study's objective was to determine the epidemiological, clinical, and laboratory characteristics of cases of invasive salmonellosis and the antibiotic susceptibility profile of salmonella isolates (2013-2017), in a series of cases of patients with Salmonella spp. isolated from secretions and/or body fluids with or without positive stool culture (n=70). For the evaluation of antibiotic susceptibility, the first isolate was considered in all cases of salmonellosis (n=168). The highest frequency of cases occurred between 0 to 4 years (14.3%) ...
Source: Revista Peruana de Medicina de Experimental y Salud Publica - Category: International Medicine & Public Health Tags: Rev Peru Med Exp Salud Publica Source Type: research
Publication date: Available online 5 December 2019Source: Journal of Global Antimicrobial ResistanceAuthor(s): Nicolás F. Cordeiro, Andrés Iriarte, Lucía Yim, Laura Betancor, José Alejandro Chabalgoity, Teresa Camou, Rafael Vignoli
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Publication date: Available online 5 December 2019Source: CellAuthor(s): Nicole Y. Lai, Melissa A. Musser, Felipe A. Pinho-Ribeiro, Pankaj Baral, Amanda Jacobson, Pingchuan Ma, David E. Potts, Zuojia Chen, Donggi Paik, Salima Soualhi, Yiqing Yan, Aditya Misra, Kaitlin Goldstein, Valentina N. Lagomarsino, Anja Nordstrom, Kisha N. Sivanathan, Antonia Wallrapp, Vijay K. Kuchroo, Roni Nowarski, Michael N. StarnbachSummaryGut-innervating nociceptor sensory neurons respond to noxious stimuli by initiating protective responses including pain and inflammation; however, their role in enteric infections is unclear. Here, we find tha...
Source: Cell - Category: Cytology Source Type: research
Publication date: Available online 5 December 2019Source: Research in Veterinary ScienceAuthor(s): Marta Perez-Sancho, Teresa García-Seco, Concepción Porrero, Nerea García, Susana Gomez-Barrero, Jose María Cámara, Lucas Domínguez, Julio ÁlvarezAbstractFeral pigeons have increased in urban settings worldwide becoming a potential health risk for humans and other animals. Control and surveillance programs are essential to prevent the possible transmission of zoonotic pathogens carried by pigeons. A surveillance program was carried out in Madrid City (Spain) during 2005–20...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Recent clusters of an uncommon strain of Salmonella traced to raw turkey products suggest that this strain may be widespread in the turkey industry.Morbidity &Mortality Weekly Report
Source: Medscape Today Headlines - Category: Consumer Health News Tags: Public Health & Prevention Journal Article Source Type: news
Conclusions: We developed a method that was capable of monitoring and quantifying amide-bonded lysine modifications in cells of different origins. PMID: 31798349 [PubMed]
Source: Biological Procedures Online - Category: Molecular Biology Authors: Tags: Biol Proced Online Source Type: research
Publication date: Available online 4 December 2019Source: Food Research InternationalAuthor(s): ZAHRA H. MOHAMMAD, ELSA A. MURANO, ROSANA G. MOREIRA, ALEJANDRO CASTILLOAbstractSince two outbreaks of salmonellosis were linked to the consumption of almonds in 2001 and 2004, the study of pathogen inactivation kinetics in almonds has been encouraged, often by conducting inoculated challenge studies. The inoculation method could affect the results of such challenge studies, because of the possible increase of moisture on the almonds resulting from a wet inoculation procedure, which may result in a potential overestimation of th...
Source: Food Research International - Category: Food Science Source Type: research
Foodborne Pathogens and Disease, Ahead of Print.
Source: Foodborne Pathogens and Disease - Category: Food Science Authors: Source Type: research
Upon intracellular cues, bacterial mRNA leaders often form secondary structures that determine expression of a downstream protein-coding region(s), thereby providing bacteria with a mechanism to control the amounts of necessary proteins in the right locales. Here we describe a polycistronic mRNA leader that secures bacterial growth by preventing dysregulated expression of the protein-coding regions. In Salmonella, the mgtCBR mRNA encodes the virulence protein MgtC and the Mg2+ transporter MgtB. A mutant designed to produce leaderless mgtCBR mRNA induced MgtC and MgtB in conditions that promote mgtC transcription. The dysre...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 2 December 2019Source: Journal of Molecular StructureAuthor(s): R. Rama, S. MeenakshiAbstractA novel Aliquat 336 based functionalized ionic liquid, methyltrioctylammonium naphthylacetate ([A336]+[NAA]-) have been synthesized and characterized using IR, UV, 1H NMR and Raman spectroscopy techniques. The prepared IL was tested for antimicrobial properties and chromium (VI) extraction. Anti-bacterial test was carried out towards five types of bacteria namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, Bacillus subtilis and Salmonella typhi. The antifungal test was done ou...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
More News: Food Science | Meat | Salmonella | Study