Biotreatment of restaurant wastewater with an oily high concentration by newly isolated bacteria from oily sludge

In this study, 75 strains from oily sludge were isolated with oil degradation activity for edible oil-contained wastewater. Eight isolates were able to grow well in liquid cultures with edible oil as the sole carbon source and discovered with high efficient oil-degrading ability. Seven out of eight isolates were identified asAcinetobacter and one isolate asKluyvera cryocrescens, based on their 16S rRNA gene sequences. Three highly efficient oil degrading bacteria (Acinetobacter dijkshoorniae LYC46-2,Kluyvera cryocrescens LYC50-1a andAcinetobacter pittii LYC73-4b) were selected and their degradation characteristic were examined, the results showed that the three isolates were effective under pH range from 7.0 to 10.0, and temperature from 25 to 35  °C. For degradation of 2–4% (v/v) of vegetable oil, >  85% degradation percentage were obtained within 30 h. Degradation of the higher concentration oil (6–8%, v/v) result in 50–70% degradation percentage within 72 h, and the degradation percentage for the isolated strains were decreased about 50% for the degradation of 10% oil (< 45%) compared to 2% oil. Different type of oils were also tested,  >  90% of degradation percentage were obtained by the three isolates, implied that these strains are capable of removing various oils efficiently. These results suggested thatAcinetobacter dijkshoorniae LYC46-2,Kluyvera cryocrescens LYC50-1a andAcinetobacter pittii LYC73-4b are potential species could be efficient...
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research