Two-dimensional membrane scaffold for the oriented immobilization of biosensing molecules

Publication date: Available online 9 November 2019Source: Biosensors and BioelectronicsAuthor(s): Masumi Iijima, Tsutomu Nakayama, Shun'ichi KurodaAbstractThe orientation and density of biosensing molecules on sensor chip should be precisely controlled to improve sensitivity and ligand-binding capacity. We previously developed a ∼30-nm bio-nanocapsule (ZZ-BNC), consisting of the hepatitis B virus envelope L protein fused with the tandem form of protein A-derived IgG Fc-binding Z domain (ZZ-L protein). This is used as a robust nanoparticle scaffold to enhance the sensitivity and ligand-binding capacity of IgGs and Fc-fused sensing molecules (Fc-fused receptors). However, due to their rigid particle structure, the surface density of ZZ-L proteins could not be optimized for biosensor functions, and useless ZZ-L proteins become stuck between ZZ-BNC and the sensor chip. Here, we have developed a planar lipid membrane embedded with ZZ-L micelles (ZZ-L membrane), which could modify the surface of any biosensor chip with a controlled density of ZZ-L proteins. Compared with ZZ-BNC, the sensitivity and ligand-binding capacity of IgGs were enhanced about 10-fold with the ZZ-L membrane. Furthermore, the immobilized IgGs could capture their respective antigens almost stoichiometrically, indicating that ZZ-L membrane is the most ideal scaffold for Fc-fused sensing molecules in terms of both clustering and oriented immobilization.
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research

Related Links:

AbstractThe aim of this work is to reveal impacts of a nuclear power plant on environmental radioactivity and public health since commission in the South China Sea. After long-term monitoring, it turns out that radioactivity in ambient air environment, water, soil, sweet potato, rice, chicken, lamb, butterfish and prawn meet demands of national environment protection standards and food safety standards in China. After 6 years ’ statistic check of local residents, the top tree diseases before and after the plant’s commission remained pulmonary tuberculosis, hand-foot-and-mouth disease, and hepatitis B. Incidence...
Source: Journal of Radioanalytical and Nuclear Chemistry - Category: Nuclear Medicine Source Type: research
This study investigated the interaction of synthesized immunogenic ‘a’ determinant (aD) region of hepatitis B virus surface antigen (HBsAg) with PAMAM G4 and anti-HBsAg antibody, as a potential bio-nanogate for anti-HBsAg detection. The aD peptide fused with maltose binding protein (MBP), was confirmed with Western blotting. Nano-Differential Scanning Fluorimetry (nano-DSF) study revealed that the interaction of MBP-aD with anti-HBsAg indicated a higher thermal stability as compared to its interaction with PAMAM G4. Electrochemical impedance spectroscopy showed that a higher binding constant of MBP-aD interacti...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Predictive value of plasmacytoid dendritic cells and Toll-like receptor-9 regarding the treatment efficacy of interferon-α in HBeAg-positive chronic hepatitis B patients. Exp Ther Med. 2019 Dec;18(6):4541-4546 Authors: Chen Y, Yang JE, Tang JM, Mao QG, Zheng QZ, Zheng Y Abstract Hepatitis B virus (HBV) infection represents a public health threat and a challenge for the medical community. Untimely treatment may lead to liver cirrhosis and even liver cancer. At present, the major treatment for hepatitis B e antigen (HBeAg)-positive chronic hepatitis B patients includes administration of interferon-...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
This study aimed to explore whether quantitative surface antigen [hepatitis B surface antigen (HBsAg)] can be used as a surrogate marker of hepatitis B virus (HBV) DNA to predict hepatitis B transmission before the first hepatitis vaccine dose in infants born to hepatitis B e antigen (HBeAg)-positive pregnant women. Background: Currently, HBV transmission persistently occurs worldwide, especially in infants born to e antigen (HBeAg)-positive highly viremic mothers. However, due to high cost, the extensive use of viral load testing to identify these high-risk mothers is limited. Materials and Methods: In total of 275 ...
Source: Journal of Clinical Gastroenterology - Category: Gastroenterology Tags: LIVER, PANCREAS & BILIARY TRACT: Original Articles Source Type: research
The title compound, C15H22N4O5S, was prepared via alkylation of 3-(chloromethyl)-5-(pentan-3-yl)-1,2,4-oxadiazole in anhydrous dioxane in the presence of triethylamine. The thiadiazine ring has an envelope conformation with the S atom displaced by 0.4883   (6)   Å from the mean plane through the other five atoms. The planar 1,2,4-oxadiazole ring is inclined to the mean plane of the thiadiazine ring by 77.45   (11) ° . In the crystal, molecules are linked by C — H...N hydrogen bonds, forming chains propagating along the b-axis direction. Hirshfeld surface analysis and two-dimensional fingerprint plots ...
Source: Acta Crystallographica Section E - Category: Chemistry Authors: Tags: crystal structure 2H-1,2,6-thiadiazine 1,1-dioxide hepatitis B HBV hydrogen bonding Hirshfeld surface analysis molecular docking study research communications Source Type: research
Source: Human Vaccines and Immunotherapeutics - Category: Allergy & Immunology Authors: Source Type: research
ConclusionsKnowledge of HBV and HCC screening recommendations is suboptimal among trainees. Efforts to broadly disseminate HBV and HCC guidelines through targeted educational interventions are needed.
Source: Digestive Diseases and Sciences - Category: Gastroenterology Source Type: research
Prior research had confirmed that biologic therapies can reactivate hepatitis B virus (HBVr) and hepatitis C in patients with psoriasis. Psoriasis patients with HBsAg-seropositive, HBeAg-seropositive, TNF- α inhibitor therapy, have a higher risk of HBVr and should be more closely monitored.
Source: Journal of the American Academy of Dermatology - Category: Dermatology Authors: Source Type: research
Publication date: Available online 6 December 2019Source: Microbial PathogenesisAuthor(s): Feng Zhu, Qiongfang Zhang, Qiongyue Zhang, Dazhi ZhangAbstractBackground and aimsRecent available treatment guidelines are pointing up clearance or seroconversion of hepatitis B e-antigen (HBeAg) as a valuable endpoint in treating HBeAg-positive chronic hepatitis B (CHB) patients. To evaluate the effect of combination therapy [interferon (IFN) plus nucleos(t)ide analogues (NAs)] versus IFN monotherapy on HBeAg seroconversion or seroclearance in HBeAg-positive CHB patients.MethodsAll available controlled clinical studies, published fr...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Condition:   Hepatitis B Virus (HBV) Interventions:   Biological: ETV/TDF+T101 No.1;   Biological: ETV/TDF+T101 No.2;   Biological: ETV or TDF;   Biological: ETV/TDF+Peg-IFNα-2b Sponsor:   Tasly Tianjin Biopharmaceutical Co., Ltd. Not yet recruiting
Source: - Category: Research Source Type: clinical trials
More News: Biotechnology | Hepatitis | Hepatitis B | Nanotechnology