Preclinical analysis of human mesenchymal stem cells: tumor tropism and therapeutic efficiency of local HSV-TK suicide gene therapy in glioblastoma.

This study analyzes the tumor homing and therapeutic abilities of clinical grade human mesenchymal stem cells (MSCs) in an orthotopic glioblastoma mouse model. Our time course analysis demonstrated that MSCs display a rapid targeted migration to intracerebral U87 glioma xenografts growing in the contralateral hemisphere within the first 48h hours after application as assessed by histology and 7T magnetic resonance imaging. MSCs accumulated predominantly peritumorally but also infiltrated the main tumor mass and targeted distant tumor satellites while no MSCs were found in other regions of the brain. Intratumoral application of MSCs expressing herpes simplex virus thymidine kinase followed by systemic prodrug application of ganciclovir led to a significant tumor growth inhibition of 86% versus the control groups (p
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research

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Authors: Wang B, Zhang J, Pan W, Cao S, Li B, Bai L, Hu P, Tian Y, Jiang D, Wang K Abstract BACKGROUND Elderly people with white-matter hyperintensities (WMHs) typically show cognitive impairment. Attention, consisting of 3 independent component processes (alerting, orienting, and executive control), is crucial for cognitive functioning. Little is known about how WMHs interfere with these attention subdomains. In the present study, we sought to describe characteristics of attention deficits in patients with age-related WMHs and to assess whether the severity and location of lesions differentially affect specific at...
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
In this study, we investigated whether the nanofibers produced by natural-synthetic polymers can probably promote the proliferation of co-cultured adipose-derived stem cells/human fibroblast cells (ADSs/HFCs) and synthesis of collagen. Nanofiber was fabricated by blending gelatin and poly (L-lactide co-ɛ-caprolactone) (PLCL) polymer nanofiber (Gel/PLCL). Cell morphology and the interaction between cells and Gel/PLCL nanofiber were evaluated by FESEM and fluorescent microscopy. MTS assay and quantitative real-time polymerase chain reaction were applied to assess the proliferation of co-cultured ADSs/HFCs and the collagen t...
Source: IET Nanobiotechnology - Category: Nanotechnology Source Type: research
Publication date: Available online 15 January 2020Source: Neuroscience &Biobehavioral ReviewsAuthor(s): Kim Fricke, Susanne VogelAbstractFRICKE, K. and S. Vogel. How interindividual differences shape approach-avoidance behavior: Relating self-report and diagnostic measures of interindividual differences to behavioral measurements of approach and avoidance. NEUROSCI BIOBEHAV REV XX(X) XXX-XXX, XXXX. -Responding to stimuli in ambiguous environments is partially governed by approach-avoidance tendencies. Imbalances in these approach-avoidance behaviors are implicated in many mental disorders including anxiety disorders, p...
Source: Neuroscience and Biobehavioral Reviews - Category: Neuroscience Source Type: research
General surgery residents encounter frequent mistreatment by patients as well as attending physicians that leads to burnout and suicidal thoughts. This is the finding of a cross-sectional national survey of general surgery residents, which found that more than 50% of general surgery residents report some form of mistreatment (N Engl J Med. 2019;381:1741-1752). Mistreatment included […]
Source: ENT Today - Category: ENT & OMF Authors: Tags: News Online Exclusives Source Type: research
Source: Psychology, Health and Medicine - Category: International Medicine & Public Health Authors: Source Type: research
A piece of the puzzle seems to be missing. The continued push by mental health practitioners and academics against the rising rates of suicide across the globe, through research and interventions in clinics and schools, has resulted in minimal success....
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
Lie-telling and secret-keeping are common behaviors during adolescence. Given the importance of honesty for building trust in positive relationships, the present study examined relations between lie-telling, secret-keeping, and relationship quality over ti...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
CONCLUSION: This research suggested that the MSCTK/GCV system exerts a strong bystander effect on GBM tumor cells, and this system may be a promising assistant method for GBM postoperative therapy. PMID: 31657679 [PubMed - as supplied by publisher]
Source: Current Gene Therapy - Category: Genetics & Stem Cells Authors: Tags: Curr Gene Ther Source Type: research
Reena Goswami1, Gayatri Subramanian2, Liliya Silayeva1, Isabelle Newkirk1, Deborah Doctor1, Karan Chawla2, Saurabh Chattopadhyay2, Dhyan Chandra3, Nageswararao Chilukuri1 and Venkaiah Betapudi1,4* 1Neuroscience Branch, Research Division, United States Army Medical Research Institute of Chemical Defense, Aberdeen, MD, United States 2Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, OH, United States 3Roswell Park Comprehensive Cancer Center, Buffalo, NY, United States 4Department of Physiology and Biophysics, Case Western Reserve University, Clev...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Previously we have shown that lentiviral vector mediated herpes simplex virus thymidine kinase (HSV-Tk) / ganciclovir (GCV) therapy is a very promising therapeutic option for GBM treatment. By using a patient derived xenograft model we have also reported complete remission on MRI after lentiviral HSV-Tk/GCV therapy. However, tumor recurrence was observed in treated animals and interestingly a fraction of recurrent glioma cells were found to be expressing tk-GFP indicating that some of the transduced cells had survived the 3-week prodrug administration period. We hypothesize that short-term prodrug delivery fails to elimina...
Source: Neuro-Oncology - Category: Cancer & Oncology Authors: Tags: EXPERIMENTAL THERAPEUTICS - PRECLINICAL STUDIES (NON-IMMUNOLOGICAL) Source Type: research
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