Role of micronize purified flavonoid fraction and ethanol Graptophyllum pictum extract on experimental anal ulcer healing. Study on Wistar rat

Publication date: Available online 6 November 2019Source: Journal of ColoproctologyAuthor(s): Sigit Adi Prasetyo, Yan Wisnu, Eriawan Agung Nugroho, Edi Dharmana, Neni Susilaningsih, Ignatius RiwantoAbstractAim of the studyTo evaluate the role of micronized purified flavanoid fraction and ethanol Graptophyllum pictum extract in the treatment of anal ulcer.MethodTwenty-eight Wistar rats were randomly allocated into four groups. Groups 2, 3 and 4 the anus were induced with croton oil, but was not induced on group 1. Groups 1 and 2 were treated with normal saline, while groups 3 and 4 were treated with micronized purified flavanoid fraction, and ethanol G. pictum extract, respectively. On 9th days blood sample were taken from the retro-orbital region, and Wistar was killed by cervical dislocation under ether anesthesia. The anal canal was resected up 2 cm from anal opening, weighted, photographically taken to measure the percentage of residual ulcer, and then prepared for microscopic examination. Elisa methods were done for SOD and MDA. The total leukocyte in the anal specimen was counted under 400 magnification power. SOD, anal coefficient, and total leukocyte for statistical analysis were using ANOVA and LSD, while MDA and percentage of ulcers were using Kruskal–Wallis and Mann–Whitney.ResultTreatment with ethanol G. pictum extract dose of 100 mg/kg BW significantly reduces the percentage of anal ulcer, the edema, leukocyte infiltration, and MDA, and increase the ...
Source: Journal of Coloproctology - Category: Gastroenterology Source Type: research