Tidy up cryo-EM sample grids with 3D printed tools

Publication date: Available online 4 November 2019Source: Journal of Structural BiologyAuthor(s): Tasuku Hamaguchi, Koji YonekuraAbstractCryo-EM technology has developed to the point of high-throughput structure determination of biological macromolecules embedded in vitreous ice. Nonetheless, challenging targets need extensive sample screening, often of many cryo-EM sample grids prepared under various conditions. We have designed and made tools for manipulating sample grids in storage cases. These tools are made of a plastic fiber using a wide-use 3D printer, a fused deposition modeling type, and polished under acetone gas. A grid case stacker organizes many frozen-hydrated cryo-EM grids and the stackers can be piled up inside a standard 50mL centrifuge tube. We have also introduced tools that facilitate handling of grid cases under liquid nitrogen and a stocker of the grid retainers contained in a CRYO ARM electron microscope. Blueprints of the tools named CryoGridTools are available from a GitHub site.Graphical abstract

Source: Journal of Structural Biology - November 6, 2019 Category: Biology Source Type: research