Removal of half antibody, hole-hole homodimer and aggregates during bispecific antibody purification using MMC ImpRes mixed-mode chromatography

Publication date: Available online 4 November 2019Source: Protein Expression and PurificationAuthor(s): Jiaqin Tang, Xudong Zhang, Tao Chen, Ying Wang, Yifeng LiAbstractDuring recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing. Among them, half antibody and homodimer are found with high frequency. In this work, with a case study we demonstrated that Capto MMC ImpRes mixed-mode chromatography can effectively remove these two by-products as well as antibody aggregates under optimized conditions. This makes MMC ImpRes a powerful tool for bsAb purification.
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
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