Diagnostic Performance of a Cytokine and IFN-γ–Induced Chemokine mRNA Assay after –Specific Antigen Stimulation in Whole Blood from Infected Individuals
Interferon (IFN)-γ release assays have limited sensitivity and cannot differentiate between active tuberculosis (TB) disease and latent TB infection (LTBI). Numerous cytokines and regulator factors have been implicated in the pathogenesis and control of Mycobacterium tuberculosis infection. Additional cytokines and chemokines associated with M. tuberculosis infection may improve the performance of IFN-γ release assays. We developed a real-time RT-PCR TaqMan assay for targeting levels of eight human targets [IFN-γ, tumor necrosis factor (TNF)-α, IL-2R, IL-4, IL-10, CXCL9, CXCL10, and CXCL11] and evaluated the assay with three different study groups.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Sunghyun Kim, Hyejon Lee, Hyunjung Kim, Yeun Kim, Jang-Eun Cho, Hyunwoo Jin, Dae Yeon Kim, Sang-Jun Ha, Young Ae Kang, Sang-Nae Cho, Hyeyoung Lee Tags: Regular article Source Type: research