Expression levels of microRNAs that are potential cytochrome P450 regulators in cynomolgus macaques.

Expression levels of microRNAs that are potential cytochrome P450 regulators in cynomolgus macaques. Xenobiotica. 2019 Nov 04;:1-21 Authors: Uno Y, Yamazaki H Abstract 1. Although the cynomolgus macaque is an important non-human primate species used in drug metabolism studies, cynomolgus macaque microRNA expressions have not been fully investigated.2. The expressions of 11 cynomolgus microRNAs, all orthologues of P450 regulators in humans, were measured by quantitative polymerase chain reaction in adrenal gland, brain, heart, jejunum, kidney, liver, ovary, testis, and uterus. mfa-miR-122 and mfa-miR-192, potentially important biomarkers for liver toxicity, were also analysed.3. Several cynomolgus microRNAs showed preferential tissue expressions: mfa-miR-1 in heart, mfa-miR-122 in liver, and mfa-miR-21 and mfa-miR-192 in jejunum. The remaining nine microRNAs had more ubiquitous expressions. All 13 cynomolgus microRNAs were expressed in liver. Among the ten animals investigated, inter-individual microRNA expression levels in liver varied from 1.5- to 5.3-fold. mfa-miR-18b was the most variable microRNA. Sex differences in expression levels were <2.0-fold, and the difference was only significant for mfa-miR-29 [1.6-fold difference (p < 0.05)]. Six cynomolgus microRNAs (mfa-miR-18b, mfa-miR-27a, mfa-miR-132, mfa-miR-27b, mfa-miR-122, and mfa-miR-29) were significantly correlated with P450 mRNAs: mfa-miR-18b and mfa-miR-27a were eac...
Source: Xenobiotica - Category: Research Authors: Tags: Xenobiotica Source Type: research