Pharmacokinetics and Metabolite Profiling of Trepibutone in Rats Using Ultra-High Performance Liquid Chromatography Combined With Hybrid Quadrupole-Orbitrap and Triple Quadrupole Mass Spectrometers

In this study, an accurate, sensitive, and selective analytical method was developed and successfully applied to assess the pharmacokinetic behavior of trepibutone in rats. Trepibutone and carbamazepine (internal standard, IS) were quantified using multiple reaction monitoring (MRM) mode with the transitions of m/z 311.09→265.08 and m/z 237.06→194.08, respectively. The linearity, precision, accuracy, extraction recovery, matrix effect, and stability of the established method were all excellent within acceptable range. A total of 30 metabolites were identified in plasma and urine by Q-Exactive high resolution mass spectrometry, and several common metabolic pathways were observed such as dealkylation, oxidation, reduction, glucuronidation, and so on. This research provides more information on trepibutone in pharmacodynamics and toxicology and will assist the usage of trepibutone in clinical.
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research

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Autoimmune pancreatitis (AIP) is a relatively newly discovered and rare disease that can be challenging to recognize and diagnose.1 Although a case report first described it in 1961, not until 1995 did it begin to be recognized as a clinical entity in a case series by Yoshida et  al.2 Type 1 AIP is the most common worldwide and is associated with IgG4-positive cells and histologic features described as lymphocytoplasmic sclerosing pancreatitis (LPSP) without granulocyte epithelial lesions, which are seen in type 2 AIP.
Source: Gastrointestinal Endoscopy - Category: Gastroenterology Authors: Tags: Original article Source Type: research
The advent of fully covered self-expandable metallic stents (FCSEMSs) represented a disruptive innovation in biliary endotherapy for benign biliary strictures. As compared with standard plastic stents, which usually require several procedures to gradually upsize the diameter of a stricture by the placement of multiple stents in parallel, the radial expansion of FCSEMSs introduced the possibility of deploying a single device that could maximally dilate the stricture. FCSEMSs are effective in cases refractory to multiple plastic stents and have comparable efficacy while reducing the number of requisite procedures when used a...
Source: Gastrointestinal Endoscopy - Category: Gastroenterology Authors: Tags: Original article Source Type: research
CONCLUSION: Urine dipstick proteinuria of 2+ or greater was significantly associated with the increased risk for incident gallstone. PMID: 31956168 [PubMed - as supplied by publisher]
Source: Journal of Epidemiology - Category: Epidemiology Tags: J Epidemiol Source Type: research
Conclusions EUS-guided PG using a small-caliber guidewire, 4F angioplasty balloon, and reverse 3F single pigtail stents offers a safe and atraumatic alternative without use of cautery. [...] © Georg Thieme Verlag KG Stuttgart · New YorkArticle in Thieme eJournals: Table of contents  |  Abstract  |  open access Full text
Source: Endoscopy International Open - Category: Gastroenterology Authors: Tags: Original article Source Type: research
This study was registered with ClinicalTrials.gov, NCT03104582, and was completed. Results Compared with the PTBD group, the ERCP group had a higher incidence of post-procedural cholangitis (37 [37.37 %] vs. 18 [22.22 %], P = 0.028) and pancreatitis (17 [17.17 %] vs. 2 [2.47 %], P = 0.001); required more salvaged biliary drainage (18 [18.18 %] vs. 5 [6.17 %], P  = 0.029), and incurred a higher cost (P 
Source: Endoscopy International Open - Category: Gastroenterology Authors: Tags: Original article Source Type: research
ConclusionBenign biliary stricture can present even decades after cholecystectomy. Roux-en-Y hepaticojejunostomy is the treatment of choice, and a long-term favorable outcome can be expected.
Source: Journal of Medical Case Reports - Category: General Medicine Source Type: research
This article discusses the work-up, diagnosis, and management of the varying pathologies that make up biliary disease including cholelithiasis, cholecystitis, biliary dyskinesia, choledocholithiasis, cholangitis, gallstone pancreatitis, and gallstone ileus. Also addressed are more challenging and rare presentations including pregnancy and bariatric anatomy.
Source: Surgical Clinics of North America - Category: Surgery Authors: Source Type: research
Abstract The prevalence of gallstones is 10% to 15% in adults. Individuals with acute cholecystitis present with right upper quadrant pain, fever, and leukocytosis. Management includes supportive care and cholecystectomy. The prevalence of choledocholithiasis is 10% to 20%, and serious complications include cholangitis and gallstone pancreatitis. The goal of management in individuals with choledocholithiasis consists of clearing common bile duct stones. Acute ascending cholangitis is a life-threatening condition involving acute inflammation and infection of the common bile duct. Treatment includes intravenous flui...
Source: Primary Care - Category: Primary Care Authors: Tags: Prim Care Source Type: research
The prevalence of gallstones is 10% to 15% in adults. Individuals with acute cholecystitis present with right upper quadrant pain, fever, and leukocytosis. Management includes supportive care and cholecystectomy. The prevalence of choledocholithiasis is 10% to 20%, and serious complications include cholangitis and gallstone pancreatitis. The goal of management in individuals with choledocholithiasis consists of clearing common bile duct stones. Acute ascending cholangitis is a life-threatening condition involving acute inflammation and infection of the common bile duct. Treatment includes intravenous fluids, analgesia, int...
Source: Primary Care: Clinics in Office Practice - Category: Primary Care Authors: Source Type: research
AbstractBackgroundSince the introduction of laparoscopic cholecystectomy (LC), there has been continued evolution in technique, instrumentation and postoperative management. With increased experience, LC has migrated to the outpatient setting. We asked whether increased availability and experience has impacted incidence of and indications for LC.MethodsThe New York (NY) State Planning and Research Cooperative System longitudinal administrative database was utilized to identify patients who underwent cholecystectomy between 1995 and 2013. ICD-9 and CPT procedure codes were extracted corresponding to laparoscopic and open ch...
Source: Surgical Endoscopy - Category: Surgery Source Type: research
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