Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method.

Analysis and Modelling of Large and Heterogeneous Populations of DNA Using a PCR-Based Method. Chembiochem. 2019 Nov 01;: Authors: Andrade H, Thomas AK, Lin W, Reddavide FV, Zhang Y Abstract The study of populations of large size and high diversity is limited by the capability of collecting data. Moreover, for a pool of individuals, each associated with a unique characteristic feature, as the pool size grows, the possible interactions increase exponentially, quickly beyond the limit of computation and experimental studies. Herein, we present designs of DNA libraries with various diversity. Using a facile analytic method based on real time PCR, we can evaluate the diversity of a pool of DNA allowing extraordinarily high heterogenicity (e.g. > 1 trillion). We demonstrated that these DNA libraries can be used to model heterogeneous populations, exhibiting functions such as self-protection, suitable for biased expansion, and to evolve into amorphous structures. The method has shown the remarkable power of parallel computing using DNA, as it can resemble an analogue computer and be applied in selection-based biotechnology methods, such as DNA-encoded chemical libraries. As a chemical approach to solve problems traditionally for genetic and statistical analysis, the method provides a quick and cost-efficient evaluation of library diversity for the intermediate steps through a selection process. PMID: 31674719 [PubMed - as suppli...
Source: Chembiochem - Category: Biochemistry Authors: Tags: Chembiochem Source Type: research