Renal ischemia/reperfusion induces a dysbalance of angiopoietins, accompanied by proliferation of pericytes and fibrosis.

Renal ischemia/reperfusion induces a dysbalance of angiopoietins, accompanied by proliferation of pericytes and fibrosis. Am J Physiol Renal Physiol. 2013 Jul 3; Authors: Khairoun M, van der Pol P, de Vries DK, Lievers E, Schlagwein N, de Boer HC, Bajema IM, Rotmans JI, van Zonneveld AJ, Rabelink TJ, van Kooten C, Reinders ME Abstract Endothelial cells (ECs) are highly susceptible to hypoxia and easily affected upon ischemia/reperfusion (I/R) during renal transplantation. Pericytes and angiopoeitins play important roles in modulating EC function. In the present study, we investigate the effect of renal I/R on dynamics of angiopoietin expression and its association with pericytes and fibrosis development. Male Lewis rats were subjected to unilateral renal ischemia for 45 minutes followed by removal of the contralateral kidney. Rats were sacrificed at different time points after reperfusion. Endothelial integrity (RECA-1),pericytes (PDGFRβ), Angiopoietin-2 (Ang-2)/Angiopoietin-1 (Ang-1) expression and interstitial collagen deposition (Sirius Red and α-SMA) were assessed using immunohistochemistry and RT-PCR. Our study shows an increase in protein expression of Ang-2 starting at 5 hours and remaining elevated up to 72 hours, with consequently higher Ang-2/Ang-1 ratio after renal I/R (p<0.05 at 48 hours). This was accompanied by an increase in protein expression of the pericytic marker PDGFRβ and a loss of ECs (both at 72 hours after I/R, p&...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research