A novel reporter gene assay for pyrogen detection.

This study explored the feasibility of a novel reporter gene assay (RGA) for pyrogen detection using RAW264.7 cells stably transfected with the NF-κB reporter gene as a pyrogenic marker. The RGA could detect different types of pyrogens, including the lipopolysaccharide of gram-negative bacteria, the lipoteichoic acid of gram-positive bacteria, and the zymosan of fungi, and a good dose-effect relationship was observed in terms of NF-κB activity. The limits of detection of the RGA to those pyrogens were 0.03 EU/ml, 0.001 μg/ml, and 1μg/ml, respectively. The method had good precision and accuracy and could be applied to many products [e.g., nivolumab, rituximab, bevacizumab, etanercept, basiliximab, Haemophilus influenza type b conjugate vaccine, 23-valent pneumococcal polysaccharide vaccine, group A and group C meningococcal conjugate vaccine, diphtheria, tetanus, pertussis (acellular, component), poliomyelitis (inactivated) vaccine, and imject alum adjuvant]. The results of this study suggest that the novel RGA has a wide pyrogen detection spectrum and is sufficiently sensitive, stable, and accurate for various applications. PMID: 31666494 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

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Condition:   Healthy Volunteers (Meningococcal Infection) Interventions:   Biological: Meningococcal Polysaccharide (Serogroups A, C, Y, and W) Tetanus Toxoid Conjugate Vaccine MenACYW conjugate vaccine;   Biological: Meningococcal (Groups A, C, Y and W 135) Oligosaccharide Diphtheria CRM197 Conjugate Vaccine;   Biological: Measles, Mumps, and Rubella Virus Vaccine L ive;   Biological: Varicella Virus Vaccine;   Biological: Pneumococcal 13-valent Conjugate Vaccine;   Biological: Diphtheria, tetanus, pertussis (acellular component), hepatitis B, poliomyelitis ...
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Discussion Vaccines are a mainstay of infectious disease prevention and health promotion. Infants, children and adults benefit from vaccines the most when they are given on the recommended schedules. However there are times when this is not possible as children come to the physician a little early, or a little late, or had unavailable records and so received addition vaccine, etc. There are many questions that arise because of these timing issues such as the one above. Standard vaccine schedules can be reviewed here. Commonly administered vaccines includes: Live-attenuated vaccines Cholera Measles, mumps, rubella (MMR ...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
lon F Abstract On 4 December 2017, French parliamentarians passed a law extending the vaccination mandates for children up to 2 years of age from three vaccinations (against diphtheria, tetanus and poliomyelitis) to 11 by adding vaccinations against pertussis, Haemophilus influenza b (Hib), hepatitis B, pneumococcal diseases, meningococcal C diseases, measles, mumps and rubella. This vote follows a recommendation made by the Steering Committee of the Citizen Consultation on Vaccination that took place in 2016. The law applies to all children born after 1 January 2018. Parents who do not fulfil the mandate will not...
Source: Euro Surveill - Category: Infectious Diseases Authors: Tags: Euro Surveill Source Type: research
Conclusion Because the mainstream media has financial interest in promoting vaccines, informed parents must seek information elsewhere about back to school vaccine mandates and exemptions. Parents, as you prepare to send your child back to school, do your homework and determine which exemptions are available in your area. A list of vaccine exemptions for all fifty states is published online by the National Vaccine Information Center. Sharing this article with other parents will also help them make informed decisions about vaccines. References: http://www.nvic.org/vaccine-laws/state-vaccine-requirements.aspx http://www.oma...
Source: vactruth.com - Category: Allergy & Immunology Authors: Tags: Top Stories truth about vaccines Vaccine Exemptions Source Type: blogs
Follow me on Twitter @drClaire Imagine there was a simple treatment that could be given to babies and toddlers that was not only remarkably effective in preventing illness, but also inexpensive. And imagine that this treatment was not only inexpensive, but also lowered overall health care costs. There’s no need to imagine; the treatment exists. It’s called immunization. It’s National Infant Immunization Week, a time to recognize and celebrate immunization. It’s during infancy that we give the most vaccines, but the benefits extend far beyond infancy and beyond those babies. The protection lasts for ...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Children's Health Health policy Infectious diseases Managing your health care Parenting Prevention Vaccines Source Type: blogs
CONCLUSION: Prevention is based on compulsory meningococcal vaccination, vaccination against seasonal influenza and pneumococcal infections for pilgrims at high risk of contracting the infection, and on vaccination against hepatitis A. Updating immunization for diphtheria/tetanus/poliomyelitis/pertussis and measles/mumps is also crucial and pilgrims must comply with hygiene precautions. PMID: 27230822 [PubMed - as supplied by publisher]
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research
Authors: Dey A, Knox S, Wang H, Beard FH, McIntyre PB Abstract This summary report on vaccine preventable diseases in Australia brings together the 3 most important national sources of routinely collected data on vaccine preventable diseases (notifications, hospitalisations and deaths) for all age groups for the period January 2008 to December 2011. The general trend towards improved control of disease is evident, particularly in the childhood years. Detailed results are available in 16 individual chapters. Although these data have limitations, which are discussed in detail in the body of the report, some clear tre...
Source: Communicable Diseases Intelligence Quarterly Report - Category: Infectious Diseases Tags: Commun Dis Intell Q Rep Source Type: research
Conclusions Dermatoses at the injection sites of vaccines can be granulomatous, immunity-related conditions, infections, lichenoid, neutrophilic, or pseudolymphomatous. Basal cell carcinoma and squamous cell carcinoma are the most common vaccination site-associated malignancies; however, melanoma and sarcomas (dermatofibrosarcoma protuberans, fibrosarcoma, and malignant fibrous histiocytoma) are also smallpox vaccine-related site neoplasms. A cutaneous immunocompromised district that is created by vaccine-induced local immunologic changes is hypothesized to be the pathogenesis of vaccination site reactions.
Source: Dermatology and Therapy - Category: Dermatology Source Type: research
Julia R. Barrett, MS, ELS, a Madison, WI–based science writer and editor, is a member of the National Association of Science Writers and the Board of Editors in the Life Sciences. About This Article open Citation: Barrett JR. 2015. Pediatric vaccines and neurodevelopment: primate study finds no adverse behavioral effects. Environ Health Perspect 123:A156; http://dx.doi.org/10.1289/ehp.123-A156 News Topics: Infectious Disease, Mental Health, Mercury, Neurologic Health Published: 1 June 2015 PDF Version (320 KB) Related EHP Article Examination of the Safety of Pediatric Vaccine Schedules in a Non-Human ...
Source: EHP Research - Category: Environmental Health Authors: Tags: Featured News Science Selection Infectious Disease June 2015 Mental Health Mercury Neurologic Health Source Type: research
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