Viruses, Vol. 11, Pages 1019: Identification of Novel Natural Products as Effective and Broad-Spectrum Anti-Zika Virus Inhibitors

Viruses, Vol. 11, Pages 1019: Identification of Novel Natural Products as Effective and Broad-Spectrum Anti-Zika Virus Inhibitors Viruses doi: 10.3390/v11111019 Authors: Gao Tai Wang Li Jiang Debnath Du Chen Zika virus (ZIKV) infection during pregnancy leads to severe congenital Zika syndrome, which includes microcephaly and other neurological malformations. No therapeutic agents have, so far, been approved for the treatment of ZIKV infection in humans; as such, there is a need for a continuous effort to develop effective and safe antiviral drugs to treat ZIKV-caused diseases. After screening a natural product library, we have herein identified four natural products with anti-ZIKV activity in Vero E6 cells, including gossypol, curcumin, digitonin, and conessine. Except for curcumin, the other three natural products have not been reported before to have anti-ZIKV activity. Among them, gossypol exhibited the strongest inhibitory activity against almost all 10 ZIKV strains tested, including six recent epidemic human strains. The mechanistic study indicated that gossypol could neutralize ZIKV infection by targeting the envelope protein domain III (EDIII) of ZIKV. In contrast, the other natural products inhibited ZIKV infection by targeting the host cell or cell-associated entry and replication stages of ZIKV. A combination of gossypol with any of the three natural products identified in this study, as well as with bortezomib, a previously reported a...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research

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By CHADI NABHAN, MD, MBA, FACP If you are a soccer fan, watching the FIFA World Cup is a ritual that you don’t ever violate. Brazilians, arguably more than any other fans in the world, live and breathe soccer—and they are always expected to be a legitimate contender to win it all. Their expectations are magnified when they are the host country, which was the case in 2014. Not only did the Germans destroy Brazilian World Cup dreams, but less than a year after a humiliating loss on their turf, Brazilians began dealing with another devastating blow: a viral epidemic. Zika left the country scrambling to understa...
Source: The Health Care Blog - Category: Consumer Health News Authors: Tags: COVID-19 Health Policy Chadi Nabhan epidemic Pandemic Zika Source Type: blogs
In this study, we asked whether ZIKV infection during pregnancy impairs transplacental transfer of IgG. We enrolled pregnant women with fever or rash in a prospective cohort in Vitoria, Brazil during the recent ZIKV epidemic. ZIKV and dengue virus (DENV)-specific IgG, ZIKV and DENV neutralizing antibodies, and routine vaccine antigen- specific IgG were measured in maternal samples collected around delivery and 20 paired cord blood samples. We concluded that 8 of these mothers were infected with ZIKV during pregnancy and 12 were ZIKV-uninfected. The magnitude of flavivirus-specific IgG, neutralizing antibody, and vaccine-el...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
In this study, we exposed apically well-differentiated human NECs cultured at the ALI to the related flaviviruses ZIKV, JEV, WNV, and Usutu virus (USUV). We selected these viruses due to the recent increasing evidences of potential threat to humans (Cadar et al., 2017; Simonin et al., 2018). We show that NECs are particularly susceptible to JEV and WNV infection and to other flaviviruses included in this study. Infection with each virus led to shedding of infectious virus particles through the apical and basolateral surfaces and triggered host mechanisms at the level of inflammatory and antiviral mediators. Given...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, we evaluated ATA as a potential antiviral drug against ZIKV replication. The antiviral activity of ATA against ZIKV replication in vitro showed median inhibitory concentrations (IC50) of 13.87 ± 1.09 μM and 33.33 ± 1.13 μM in Vero and A549 cells, respectively; without showing any cytotoxic effect in both cell lines (median cytotoxic concentration (CC50)> 1,000 μM). Moreover, ATA protected both cell types from ZIKV-induced cytopathic effect (CPE) and apoptosis in a time- and concentration-dependent manner. In addition, pre-treatment of Vero cells with ATA for up to 72 h also resulted...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusions Apart West Nile virus (78), thousands of congenital microcephaly cases, fetal brain tissue damage and neurological syndromes have been associated with ZIKV infection. Unfortunately, the epidemics of this mosquito born, and a relative stable virus is on a rise. Although congenital microcephaly is a rare disorder however, due to lack of standardized diagnostic test facilities, the incidence in the geographically widespread ZIKV epidemic regions is higher. Animals studies showed that ZIKV is a neurotropic virus. It directly targets the developing embryonic brain cells by inducing apoptosis, cell-cycle arrest, and...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
We described ZIKV and DENV serological markers and the maternal-fetal t ransfer of antibodies among mothers and neonates after the ZIKV microcephaly outbreak in Northeast Brazil (2016). We included 89 microcephaly cases and 173 neonate controls at time of birth and their mothers. Microcephaly cases were defined as newborns with a particular head circumference (2 SD belo w the mean). Two controls without microcephaly were matched by the expected date of delivery and area of residence. We tested maternal serum for recent (ZIKV genome, IgM and IgG3 anti-NS1) and previous (ZIKV and DENV neutralizing antibodies [NAbs]) markers ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Analysis of community where 73% of residents contracted Zika in 2015 offers new clues about epidemicScientists studying the 2015 Zika outbreak in Brazil have discovered that people previously exposed to dengue may have been protected from the virus.Three-quarters of the inhabitants of a favela in the country ’s north-east caught the mosquito-borne Zika virus during the epidemic.The outbreak left more than 3,000 babies across Brazil with microcephaly, a birth defect caused by mothers catching the virus during pregnancy.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Global health Zika virus Global development Brazil Infectious diseases Science Americas World news Source Type: news
Maternal Zika virus (ZIKV) infection during pregnancy is recognized as the cause of an epidemic of microcephaly and other neurological anomalies in human fetuses. It remains unclear how ZIKV accesses the highly vulnerable population of neural progenitors of the fetal central nervous system (CNS), and which cell types of the...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Biological Sciences Source Type: research
This article describes the laboratory based surveillance system in Suriname and the incidence of confirmed cases of ZIKV infection according to sex, age, and spatial and temporal distribution Methods General practitioners and public health centers located in different districts of Suriname were asked to send blood samples from suspicious cases to Academic Hospital for molecular diagnosis of Zika virus infection. In particular, in cases involving pregnant women, samples were screened for free upon request. Viral RNA was extracted from blood and urine samples using a RNeasy Mini Kit (Qiagen, Hilden, Germany). An in-house ...
Source: PLOS Currents Outbreaks - Category: Epidemiology Authors: Source Type: research
Conclusions Surveillance, risk assessment, and intervention were strengthened throughout Malaysia in response to the 2016 outbreak of ZIKV in neighboring Singapore. The Malaysian Ministry of Health undertook regular surveillance from June 2015 during the South American outbreak, but no ZIKV was detected in 784 samples tested up to August 2016 (https://kpkesihatan.com/2016/08/28/kenyataan-akhbar-kpk-28-ogos-2016-situasi-terkini-virus-zika-di-malaysia/). However, during the peak of the 2016 Singapore ZIKV outbreak from September through December 2016, eight out of 849 samples tested were positive for ZIKV (https://kpkesiha...
Source: PLOS Currents Outbreaks - Category: Epidemiology Authors: Source Type: research
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