Characterization and immunostimulatory activity of extracellular vesicles from Filifactor alocis

In this study, we purified and characterized the protein profiles of EVs fromF. alocis and investigated their immunostimulatory activity on human monocytic THP ‐1 and human oral keratinocyte HOK‐16B cell lines. Highly pure EVs were obtained fromF. alocis using density gradient ultracentrifugation. Nanoparticle tracking analysis and transmission electron microscopy showed thatF. alocis EVs were between 50 nm and 270 nm in diameter. Proteome analysis identified 28 proteins, including lipoproteins, autolysins,F. alocis complement inhibitor (FACIN), transporter ‐related proteins, metabolism‐related proteins, and ribosomal proteins. Human cytokine array analysis showed thatF. alocis EVs remarkably induced the expression of CCL1, CCL2, MIP ‐1, CCL5, CXCL1, CXCL10, ICAM‐1, IL‐1β, IL‐1ra, IL‐6, IL‐8, MIF, SerpinE, and TNF‐α in THP‐1 cells and CXCL1, G‐CSF, GM‐CSF, IL‐6, and IL‐8 in HOK‐16B cells. The immunostimulatory activity ofF. alocis EVs was similar to that of the whole bacterial cells. Our findings provide new insight into the role of EVs from Gram ‐positive oral bacteria in periodontal diseases.
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research