Photochemical regeneration of flavoenzymes – An Old Yellow Enzyme case-study

Publication date: Available online 31 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): M.C.R. Rauch, M. Pesic, M.M.E. Huijbers, M. Pabst, C.E. Paul, M. Pesic, I.W.C.E. Arends, F. HollmannAbstractDirect, NAD(P)H-independent regeneration of Old Yellow Enzymes represents an interesting approach for simplified reaction schemes for the stereoselective reduction of conjugated C=C-double bonds. Simply by illuminating the reaction mixtures with blue light in the presence of sacrificial electron donors enables to circumvent the costly and unstable nicotinamide cofactors and a corresponding regeneration system.In the present study, we characterise the parameters determining the efficiency of this approach and outline the current limitations. Particularly, the photolability of the flavin photocatalyst and the (flavin-containing) biocatalyst represent the major limitation en route to preparative application.

Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 1, 2019 Category: Biochemistry Source Type: research