Desmoglein-3 acts as a pro-survival protein by suppressing reactive oxygen species and doming whilst augmenting the tight junctions in MDCK cells
In this study, we explored the role of Dsg3 on dome formation, reactive oxygen species (ROS) production and transepithelial electrical resistance (TER) in MDCK cells, a kidney epithelial cell model widely used to study cell differentiation and tight junction formation and integrity. We show that overexpression of Dsg3 constrained nuclear ROS production and cellular doming in confluent cell cultures and these features coincided with augmented TER and enhanced tight junction integrity. Conversely, cells expressing dominant-negative Dsg3ΔC mutants exhibited heightened ROS production and accelerated doming, accompanied by increased apoptosis, as well as cell proliferation, with massive disruption in F-actin organization and accumulation and alterations in tight junctions. Inhibition of actin polymerization and protein synthesis was able to sufficiently block dome formation in mutant populations. Taken together, these findings underscore that Dsg3 has a role in controlling cellular viability and differentiation as well as the functional integrity of tight junctions in MDCK cells.
Authors: Shimada H, Shimada Y Abstract An 86-year-old man, who had undergone a lumboperitoneal shunt for idiopathic normal pressure hydrocephalus (iNPH) implanted 4 years earlier showed progressive parkinsonism for the past year. His clinical symptoms, including resting tremor and rapid eye movement sleep behavior disorder, responsiveness to levodopa, and abnormal findings on 123I-MIBG myocardial scintigraphy and dopamine transporter imaging, indicated that his pathological background of parkinsonism included concomitant synucleinopathy, such as Parkinson's disease or dementia with Lewy bodies, in addition to iNPH....
Publication date: Available online 29 May 2020Source: Neurología (English Edition)Author(s): F. Valldeoriola, F. Grandas, J.M. Arbelo, M. Blázquez Estrada, M. Calopa Garriga, V.M. Campos-Arillo, P.J. Garcia Ruiz, J.C. Gómez Esteban, C. Leiva Santana, J.C. Martínez Castrillo, P. Mir, A. Salvador Aliaga, F. Vivancos Matellano, R.M. Yáñez Baña
Conclusions: The current study indicates that TG infection could improve the cataleptic, asymmetric, nociceptive and behaviors; the level of striatal dopamine release; BDNF levels; TAC; and MDA in PD rats. PMID: 32461971 [PubMed - in process]
Effects of Cardiac Sympathetic Neurodegeneration and PPARγ Activation on Rhesus Macaque Whole Blood miRNA and mRNA Expression Profiles. Biomed Res Int. 2020;2020:9426204 Authors: Metzger JM, Lopez MS, Schmidt JK, Murphy ME, Vemuganti R, Emborg ME Abstract Degeneration of sympathetic innervation of the heart occurs in numerous diseases, including diabetes, idiopathic REM sleep disorder, and Parkinson's disease (PD). In PD, cardiac sympathetic denervation occurs in 80-90% of patients and can begin before the onset of motor symptoms. Today, there are no disease-modifying therapies for cardiac sympa...
PARKINSON'S disease predominantly affects a certain region in the brain. As the condition gradually becomes apparent, what's the one change in bowel habits that may lead to an earlier diagnosis?
Publication date: Available online 28 May 2020Source: Stem Cell ReportsAuthor(s): Akihiro Yamaguchi, Kei-ichi Ishikawa, Tsuyoshi Inoshita, Kahori Shiba-Fukushima, Shinji Saiki, Taku Hatano, Akio Mori, Yutaka Oji, Ayami Okuzumi, Yuanzhe Li, Manabu Funayama, Yuzuru Imai, Nobutaka Hattori, Wado Akamatsu
Publication date: September 2020Source: Neurochemistry International, Volume 138Author(s): Francesco Dernie
Although nilotinib is safe and tolerable in patients with moderate or advanced Parkinson's disease, it may not affect the disease's symptoms, according to investigators.Medscape Medical News
Condition: Parkinson Disease Intervention: Other: Cycling deep brain stimulation Sponsor: University of Sao Paulo General Hospital Not yet recruiting
The authors regret not having included the name of Camilla Zamfolini Hallal as a co-author of the referred article at the appropriate time. However, in view of the important contributions of the co-author to the article, we request this misunderstanding to...