Comparison of 2D and 3D cell culture models for cell growth, gene expression and drug resistance

Publication date: February 2020Source: Materials Science and Engineering: C, Volume 107Author(s): Julia C. Fontoura, Christian Viezzer, Fabiana G. dos Santos, Rosane A. Ligabue, Ricardo Weinlich, Renato D. Puga, Dyeison Antonow, Patricia Severino, Cristina BonorinoAbstractIn vitro drug screening is widely used in the development of new drugs, because they constitute a cost-effective approach to select compounds with more potential for therapy. They are also an attractive alternative to in vivo testing. However, most of these assays are done in two-dimensional culture models, where cells are grown on a polystyrene or glass flat surface. In order to develop in vitro models that would more closely resemble physiological conditions, three-dimensional models have been developed. Here, we introduce two novel fully synthetic scaffolds produced using the polymer polyhydroxybutyrate (PHB): a Solvent-Casting Particle-Leaching (SCPL) membrane; and an electrospun membrane, to be used for 3D cultures of B16 F10 murine melanoma cells and 4T1 murine breast cancer cells. A 2D cell culture system in regular tissue culture plates and a classical 3D model where cells are grown on a commercially available gel derived from Engelbreth-Holm Swarm (EHS) tumor were used for comparison with the synthetic scaffolds. Cells were also collected from in vivo tumors grown as grafts in syngeneic mice. Morphology, cell viability, response to chemotherapy and gene expression analysis were used to compare all s...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research