Molecular diversity and antibiotic resistance gene profile of Salmonella enterica serovars isolated from humans and food animals in Lagos, Nigeria.

This study determined the diversity and antibiotic resistance gene profile of Salmonella enterica serovars isolated from humans and food animals. Using standard methods, Salmonella spp. were isolated from fecal samples, profiled for antimicrobial susceptibility and resistance genes. Seventy-one Salmonella isolates were recovered from both humans and food animals comprising cattle, sheep, and chicken. Forty-four serovars were identified, with dominant Salmonella Budapest (31.8%). Rare serovars were present in chicken (S. Alfort, S. Wichita, S. Linton, S. Ealing, and S. Ebrie) and humans (S. Mowanjum, S. Huettwillen, S. Limete, and S. Chagoua). Sixty-eight percent of isolates were sensitive to all test antibiotics, while the highest rate of resistance was to nalidixic acid (16.9%; n = 12), followed by ciprofloxacin (11.3%; n = 8) and tetracycline (9.9%; n = 8). Five isolates (7%) were multidrug-resistant and antimicrobial resistance genes coding resistance to tetracycline (tetA), beta-lactam (blaTEM), and quinolone/fluoroquinolone (qnrB and qnrS) were detected. Evolutionary analysis of gyrA gene sequences of human and food animal Salmonella isolates revealed variations but are evolutionarily interconnected. Isolates were grouped into four clades with S. Budapest isolate from cattle clustering with S. Budapest isolated from chicken, whereas S. Essen isolated from sheep and chicken was grouped into a clade. Diverse S. enterica serovars wi...
Source: Acta Microbiologica et Immunologica Hungarica - Category: Microbiology Authors: Tags: Acta Microbiol Immunol Hung Source Type: research

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Conclusion This study revealed that Enterococcus species with biofilm potentials and extracellular virulence properties extensively occur in retail RTE shrimps. A significant number of isolated strains are resistant to antibiotics and harbor resistant and virulent genes, denoting a significant route of resistance and virulence dissemination to bacteria in humans. There is an inadequate understanding of the intricacies of antibiotic-resistant enterococci of food origin that belong to enterococci aside from E. faecium and E. faecalis. Findings from this study reveal detailed antibiotic resistance of E. durans, E. casselifla...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study revealed that a diverse group of plasmids, carrying AR genes, are responsible for the phenotypic resistance seen in Salmonella isolated from United States food animals. It was also determined that many plasmids carry similar ARCs. Introduction Non-typhoidal Salmonella enterica is one of the most common causes of foodborne illnesses globally, with an estimated 1.2 million cases each year in the United States alone (CDC, 2013). Symptoms range from self-limiting gastrointestinal illness to sepsis. These infections can lead to death unless treated with antibiotics (Crump et al., 2015). Unfortunately, antimic...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, a “species” is defined as a unique AMR profile, including the profile corresponding to susceptibility to all tested antimicrobials. The AMR profile (or antibiogram) of an isolate is the combination of AMR phenotype (susceptible or non-susceptible) to each drug tested. SR reflects the richness of AMR profiles without weighting of abundance. SE and SD are measures in which both SR and relative abundance are taken into account. The BP diversity index is related to the proportion of isolates with the most common AMR profile. Changes in antimicrobial testing protocol could impact upon results, partic...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion In this study, a self-transmissible IncN2 plasmid bearing blaNDM was detected in ST405 E. coli, which is a globally disseminated lineage. We highlight the dissemination potential of IncN2 plasmids harboring blaNDM. The presence of blaNDM mediated by plasmids in the high-risk clone ST405 E. coli linked with multiple virulence genes and resistance genes is increasing, which may create health concerns. Author Contributions YJ and ZL designed the experiments. YH analyzed the data and wrote the manuscript. CS and XG prepared the table and figures. YB carried out the bacteria identification. Funding The study was...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, association rule mining is an effective tool for identifying patterns of multidrug resistance within antimicrobial susceptibility testing data and evaluating the statistical and biological significance of the patterns. Rule quality measures used to sort and differentiate rules should be tested using resampling procedures to minimize the false discovery rate. Rule mining identified consistent multidrug resistance patterns involving beta-lactams, sulfisoxazole, tetracycline, gentamicin, and streptomycin in E. coli isolated from chicken carcasses and meat between 2004 and 2012. The generally low rule overlap su...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The objective of this research was to determine changes to lettuce phyllosphere microbiota, inoculated ARB, and the resistome (profile of ARGs) following washing with a sanitizer, gamma irradiation, and cold storage. To simulate potential sources of pre-harvest contamination, romaine lettuce leaves were inoculated with compost slurry containing antibiotic-resistant strains of pathogenic (Escherichia coli O157:H7) and representative of spoilage bacteria (Pseudomonas aeruginosa). Various combinations of washing with sodium hypochlorite (50 ppm free chlorine), packaging under modified atmosphere (98% nitrogen), irradiating (1...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
ConclusionsAntimicrobial resistance rates in Salmonella serotypes from broiler chicken farms were alarming, especially for ciprofloxacin and azithromycin. Thus, another therapeutic strategy other than antimicrobials is recommended to prevent outbreaks of MDR Salmonella.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Publication date: Available online 24 July 2018Source: Food ControlAuthor(s): Aihua Zhu, Wei Zhi, Yongfeng Qiu, Lingling Wei, Jun Tian, Zhiming Pan, Xilong Kang, Wenlong Gu, Liqin DuanAbstractSalmonella is considered one of the most important foodborne pathogens and is commonly associated with the consumption of pork and other animal products. Multidrug resistant (MDR) Salmonella strains are highly adaptive and have been responsible for foodborne disease outbreaks. We investigated Salmonella prevalence and antimicrobial resistance in pork obtained from open markets in Xuzhou, Jiangsu Province, China. From October 2016 to S...
Source: Food Control - Category: Food Science Source Type: research
Conclusions the resistance rates in Salmonella serovars in broiler chicken farms were alarming, especially for ciprofloxacin and azithromycin. Thus, another therapeutic strategy other than antibiotics is recommended to prevent the outbreaks of multidrug resistant salmonellae.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
In this study, we identify temporal trends in the distribution of Salmonella serotypes and their mechanisms of antimicrobial resistance; further, we are able to determine the likely origin of several temporal clusters of infection by using whole genome sequencing. These data can be used to focus strategies to better contain the dissemination and enhance the mitigation of Salmonella infections, and to provide evidence-based policies and guidelines to steward antimicrobial use in veterinary medicine. PMID: 29678910 [PubMed - as supplied by publisher]
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
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