Use of nucleoside phosphorylases for the preparation of 5-modified pyrimidine ribonucleosides

Publication date: Available online 30 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Cyril S. Alexeev, Mikhail S. Drenichev, Evgeniya O. Dorinova, Roman S. Esipov, Irina V. Kulikova, Sergey N. MikhailovAbstractEnzymatic transglycosylation, a transfer of the carbohydrate moiety from one heterocyclic base to another, is catalyzed by nucleoside phosphorylases (NPs) and is being actively developed and applied for the synthesis of biologically important nucleosides. Here, we report an efficient one-step synthesis of 5-substitited pyrimidine ribonucleosides starting from 7-methylguanosine hydroiodide in the presence of nucleoside phosphorylases (NPs).Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - Category: Biochemistry Source Type: research