Viruses, Vol. 11, Pages 996: Survivin Overexpression Has a Negative Effect on Feline Calicivirus Infection

Viruses, Vol. 11, Pages 996: Survivin Overexpression Has a Negative Effect on Feline Calicivirus Infection Viruses doi: 10.3390/v11110996 Authors: Oscar Salvador Barrera-Vázquez Clotilde Cancio-Lonches Carlos Emilio Miguel-Rodríguez Monica Margarita Valdes Pérez Ana Lorena Gutiérrez-Escolano It is known that levels of the anti-apoptotic protein survivin are reduced during Murine norovirus MNV-1 and Feline calicivirus (FCV) infection as part of the apoptosis establishment required for virus release and propagation in the host. Recently, our group has reported that overexpression of survivin causes a reduction of FCV protein synthesis and viral progeny production, suggesting that survivin may affect early steps of the replicative cycle. Using immunofluorescence assays, we observed that overexpression of survivin, resulted in the reduction of FCV infection not only in transfected but also in the neighboring nontransfected CrFK cells, thus suggesting autocrine and paracrine protective effects. Cells treated with the supernatants collected from CrFK cells overexpressing survivin showed a reduction in FCV but not MNV-1 protein production and viral yield, suggesting that FCV binding and/or entry were specifically altered. The reduced ability of FCV to bind to the surface of the cells overexpressing survivin, or treated with the supernatants collected from these cells, correlate with the reduction in the cell surface of the FCV receptor, the feline junctional adhe...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research