GeTFEP: A general transfer free energy profile of transmembrane proteins

In this study, we derived a TFE profile named General Transfer Free Energy Profile (GeTFEP) based on computation of the TFE s of 58β‐barrel membrane proteins (βMPs). The GeTFEP agrees well with experimentally measured and computationally derived TFEs. Analysis based on the GeTFEP shows that residues in different regions of the TM segments ofβMPs have different roles during the membrane insertion process. Results further reveal the importance of the sequence pattern of transmembrane strands in stabilizingβMPs in the membrane environment. In addition, we show that GeTFEP can be used to predict the positioning and the orientation ofβMPs in the membrane. We also show that GeTFEP can be used to identify structurally or functionally important amino acid residue sites ofβMPs. Furthermore, the TM segments ofα‐helical membrane proteins can be accurately predicted with GeTFEP, suggesting that the GeTFEP is of general applicability in studying membrane protein.
Source: Protein Science - Category: Biochemistry Authors: Tags: Full ‐Length Papers Source Type: research