Baby-SPIME: A dynamic in vitro piglet model mimicking gut microbiota during the weaning process

Publication date: Available online 25 October 2019Source: Journal of Microbiological MethodsAuthor(s): Sandrine Dufourny, Nadia Everaert, Sarah Lebrun, Caroline Douny, Marie-Louise Scippo, Li Bing, Bernard Taminiau, Massimo Marzorati, José Wavreille, Eric Froidmont, Pierre Rondia, Véronique DelcenserieAbstractThe study aimed to adapt the SHIME® model, developed to simulate human digestion and fermentation, to a baby-SPIME (baby Simulator of Pig Intestinal Microbial Ecosystem). What constitutes a unique feature of this model is its twofold objective of introducing an ileal microbial community and mimicking a dietary weaning transition. This model should then be ideally suited to test the dietary weaning strategies of piglets in vitro. Regarding the microbiota, the main phyla making up the model were Firmicutes, Bacteroidetes and Proteobacteria although Bacteroidetes decreased after inoculation (p = 0.043 in ileum, p = 0.021 in colon) and Delta-Proteobacteria were favoured (p = 0.083 in ileum, p = 0.043 in colon) compared to Gamma-Proteobacteria. The designed model led to a low representation of Bacilli - especially Lactobacillus sp. in the ileum and a weak representation of Bacteroidia in the proximal colon. However, Mitsuokella and Prevotella were part of the major genera of the model along with Bifidobacterium, Fusobacterium, Megasphaera and Bacteroides. As a result of weaning, two major changes - normally occurring in vivo - were detected i...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research

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