Comparison of Real-Time quantitative PCR and digital droplet PCR for BCR-ABL1 Monitoring in Patients with Chronic Myeloid Leukemia

Real-time quantitative PCR (RT-qPCR) is routinely used to detect minimal residual disease in chronic myeloid leukemia patients. The absolute quantification with droplet digital PCR (dPCR) could reduce the inherent variability of RT-qPCR. We established a duplex dPCR assay using the EAC primer/probe system for BCR-ABL1 and ABL1 and compared the results to RT-qPCR. Two-hundred and thirty cDNA samples from patients with chronic myeloid leukemia were analyzed using both procedures. A second, commercially developed dPCR assay for BCR-ABL1 was also evaluated.

Source: Journal of Molecular Diagnostics - October 23, 2019 Category: Pathology Authors: Georg-Nikolaus Franke, Jacqueline Maier, Kathrin Wildenberger, Michael Cross, Francis J. Giles, Martin C. M üller, Andreas Hochhaus, Dietger Niederwieser, Thoralf Lange Tags: Regular Article Source Type: research