Using nanopore RNA-Seq to HLA genotype and correlate donor HLA expression with flow cytometric crossmatch results

Transplant centers are increasingly using virtual crossmatching (VXM) to evaluate recipient and donor compatibility. However, the current state of VXM fails to incorporate donor HLA expression in the assessment, despite numerous studies that demonstrated the impact of donor HLA expression on physical crossmatch outcomes. Whole-transcriptome sequencing (RNA-Seq) for HLA enables simultaneous determination of HLA genotyping and relative HLA expression. Ultimately the RNA-Seq needs to be faster to incorporate into the VXM process.

Source: Journal of Molecular Diagnostics - October 23, 2019 Category: Pathology Authors: Maureen C. Montgomery, Chang Liu, Rosanne Petraroia, Eric T. Weimer Tags: Regular Article Source Type: research