The JAK inhibitor tofacitinib ameliorates immune ‑mediated liver injury in mice.

The JAK inhibitor tofacitinib ameliorates immune‑mediated liver injury in mice. Mol Med Rep. 2019 Oct 16;: Authors: Wang H, Feng X, Han P, Lei Y, Xia Y, Tian D, Yan W Abstract The prevalence of immune‑mediated liver diseases such as autoimmune liver disease or viral hepatitis has increased in recent years, and the side effects of pre‑existing treatments are a worldwide problem. Regulatory T cells (Tregs) and T helper 17 (Th17) cells play important roles in the development of immune‑mediated hepatitis and may serve as potential therapeutic targets. Tofacitinib, a new Janus kinase (JAK) inhibitor, is under investigation for the treatment of rheumatoid arthritis; it is also helpful in treating ulcerative colitis and psoriasis. The roles of tofacitinib were investigated in conferring protection against immune‑mediated liver injury in mice. T cell‑mediated hepatitis was induced by concanavalin A (ConA). The mice in the treatment groups were administered with tofacitinib intragastrically before the ConA injection. Histopathological examination was performed by hematoxylin and eosin (H&E) staining, and the serum transaminase and inflammatory cytokine levels were determined using an automatic biochemistry analysis apparatus or cytometric bead array (CBA) kits. Flow cytometric analysis was used to detect Tregs and Th17 cells. Tofacitinib significantly decreased the hepatic injury induced by ConA and prominently decreased th...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research