Nucleolin Mediates the Binding of Cancer Cells to L-selectin under Conditions of Lymphodynamic Shear Stress.

Nucleolin Mediates the Binding of Cancer Cells to L-selectin under Conditions of Lymphodynamic Shear Stress. Am J Physiol Cell Physiol. 2019 Oct 23;: Authors: Goldson TM, Turner KL, Huang Y, Carlson GE, Caggiano EG, Oberhauser AF, Fennewald SM, Burdick MM, Resto VA Abstract Head and neck squamous cell carcinoma (HNSCC) cells bind to lymphocytes via L-selectin in a shear-dependent manner. This interaction takes place exclusively under low shear stress conditions, such as those found within the lymph node parenchyma. This represents a novel functional role for L-selectin/selectin ligand interactions. Our previous work has characterized as of yet unidentified L-selectin ligands expressed by HNSCC cells that are specifically active under conditions of low shear stress consistent with lymph flow. Using an affinity purification approach, we now show that nucleolin expressed on the cell surface of HNSCC cells is an active ligand for L-selectin. Parallel plate chamber flow based experiments and atomic force microscopy (AFM) experiments show that nucleolin is the main functional ligand in these low force conditions. Furthermore, AFM shows a clear relationship between work of de-adhesion and physiological loading rates. Our results reveal nucleolin as the first major ligand reported for L-selectin that operates in low shear stress conditions. PMID: 31644306 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research