Manganese ‐doped green tea‐derived carbon quantum dots as a targeted dual imaging and photodynamic therapy platform

Fluorescent manganese ‐doped carbon quantum dot derived from waste green tea and functionalized with folic acid and chlorin e6 can act as dual‐modal fluorescence/MRI probes and photodynamic therapeutic agents to detect and treat cancers. AbstractIn this work, manganese ‐doped carbon quantum dots (Mn‐CQDs) have been synthesized through a one‐pot hydrothermal method by using waste green tea. The Mn2+ dopants were introduced to impart magnetic resonance capability. Upon optimization of the experimental conditions, magnetofluorescent Mn ‐CQDs exhibit an excitation‐dependent blue emission. The abundant functional groups on Mn‐CQDs not only promote water solubility but also allow straightforward functionalization with amine groups. The amine‐terminated Mn‐CQDs were then subsequently conjugated to folic acid (FA) and chlorin e6 (Ce6) to obtain the Mn‐CQDs@FA/Ce6 magnetofluorescent photodynamic therapy (PDT) agent. in vitro studies using three different cells indicated specific targeting of Mn‐CQDs@FA/Ce6 to the overexpressing folate receptor human epithelial carcinoma cell line (HeLa) cancer cells. Furthermore, Mn‐ CQDs@FA/Ce6 enhanced magnetic resonance imaging (MRI) signal with anr2/r1 ratio of 5.77. Favorably, by using the Mn ‐CQDs@FA delivery system, active Ce6 can reach the cellular interior while its red fluorescence (FL) and reactive oxygen species generation can be retained, as has been verified by confocal microscopy. in vitro cell viability stud...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: ORIGINAL RESEARCH REPORT Source Type: research