Pilot Evaluation of a Fully Automated Bioinformatics System for Analysis of Methicillin-Resistant Staphylococcus aureus Genomes and Detection of Outbreaks Bacteriology

Genomic surveillance that combines bacterial sequencing and epidemiological information will become the gold standard for outbreak detection, but its clinical translation is hampered by the lack of automated interpretation tools. We performed a prospective pilot study to evaluate the analysis of methicillin-resistant Staphylococcus aureus (MRSA) genomes using the Next Gen Diagnostics (NGD) automated bioinformatics system. Seventeen unselected MRSA-positive patients were identified in a clinical microbiology laboratory in England over a period of 2 weeks in 2018, and 1 MRSA isolate per case was sequenced on the Illumina MiniSeq instrument. The NGD system automatically activated after sequencing and processed fastq folders to determine species, multilocus sequence type, the presence of a mec gene, antibiotic susceptibility predictions, and genetic relatedness based on mapping to a reference MRSA genome and detection of pairwise core genome single-nucleotide polymorphisms. The NGD system required 90 s per sample to automatically analyze data from each run, the results of which were automatically displayed. The same data were independently analyzed using a research-based approach. There was full concordance between the two analysis methods regarding species (S. aureus), detection of mecA, sequence type assignment, and detection of genetic determinants of resistance. Both analysis methods identified two MRSA clusters based on relatedness, one of which contained 3 cases that were i...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Bacteriology Source Type: research

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Publication date: Available online 15 January 2020Source: Aggression and Violent BehaviorAuthor(s): Tobore Onojighofia ToboreAbstractAlcohol use is known to result in disinhibition. However, evidence suggests that alcohol consumption could also result in inhibition. Several hypotheses have been proposed for this confounding alcohol-induced behavior, but none explains it adequately at the level of the brain. In this paper, oxidative stress is put forth as the underlying cause of alcohol toxicity and behavioral impairments. The implications of this understanding and how oxidative stress influences behavior in obesity, diabet...
Source: Aggression and Violent Behavior - Category: Psychiatry & Psychology Source Type: research
The vaping illness epidemic may be slowing two weeks after the FDA announced it will enact a ban on flavored e-cigarettes and New Jersey lawmakers inch toward their own measure.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Authors: Sánchez-DelaCruz E, Weber R, Biswal RR, Mejía J, Hernández-Chan G, Gómez-Pozos H Abstract Machine learning, one of the core disciplines of artificial intelligence, is an approach whose main emphasis is analytical model building. In other words, machine learning enables an automaton to make its own decisions based on a previous training process. Machine learning has revolutionized every research sector, including health care, by providing precise and accurate decisions involving minimal human interventions through pattern recognition. This is emphasized in this research, which ad...
Source: Computational and Mathematical Methods in Medicine - Category: Statistics Tags: Comput Math Methods Med Source Type: research
The 69-year-old man died in the early hours of January 15 at Jinyintan Hospital in Wuhan city, where 41 patients have fallen ill since December.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
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Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
Authors: Lu M, Yi T, Xiong Y, Wang Q, Yin N Abstract Cortex Mori Radicis extract (CMR) has various pharmacological properties, such as anti‑inflammatory, anti‑allergic and anti‑hyperglycemic effects. However, the effects and mechanisms of CMR in the neuroregeneration of diabetic peripheral neuropathy (DPN) are unclear. In the present study, the effects of CMR on neurite outgrowth of dorsal root ganglia (DRG) neurons in diabetic rats were investigated and its underlying mechanisms were explored. SD rats were subjected to a high‑fat diet with low‑dose streptozotocin to induce a Type II diabetes model with p...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
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Source: Medscape Diabetes Headlines - Category: Endocrinology Tags: Pediatrics News Source Type: news
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Source: American Heart Journal - Category: Cardiology Source Type: research
Candida auris has recently emerged as a global cause of multidrug resistant fungal outbreaks. An outbreak occurred at a tertiary care center in London in 2016. Transmission characteristics, interventions, patient outcomes and cost of resources are described.
Source: Current Awareness Service for Health (CASH) - Category: Consumer Health News Source Type: news
Pneumococcal outbreaks are rare but they still occur, particularly in closed settings usually involving vulnerable groups. The authors undertook a systematic review to identify strategies for controlling pneumococcal outbreaks since the licensure of higher-valent pneumococcal conjugate vaccines (PCVs).
Source: Current Awareness Service for Health (CASH) - Category: Consumer Health News Source Type: news
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